Addgene

Showing: 7821 - 7840 of 18787 results

Results per page:

Sort results by:

pDONR221-RAP1

Plasmid

#84525

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Tags

Expression

Mutation

no stop codon

Promoter

none

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
(PD-S608A)FLAG-Cep215-FL

Plasmid

#106905

Purpose

Expresses murine non-phosphorylatable Cep215 at S608

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

CEP215 (Cdk5rap2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine-608 to Alanine

Promoter

CMV

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-myc-mIFITM3

Plasmid

#58458

Purpose

Expresses murine IFITM3 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only
pIK137

Plasmid

#65632

Purpose

Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter and contains a C. briggsae unc-119 rescuing fragment

Depositor

Iskra Katic

Article

Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

Insert

Peft-3::gfp::h2b::tbb-2 3'UTR, C. briggsae unc-119 rescue fragment

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Dll3ICD-HPC4 pBacPAK8-3

Plasmid

#17334

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Delta-like 3 (Dll3 Mouse)

Use

Tags

HPC4

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pCRII-Sema4d-ISH-2kb

Plasmid

#68032

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4D (Sema4d Mouse)

Use

Mrna in situ hybridization probe

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-Plxnb1-ISH-2kb

Plasmid

#68035

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B1 (Plxnb1 Mouse)

Use

Mrna in situ hybridization probe

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PCDF1-ERBB4 (E872K)

Plasmid

#29543

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST53-CypB-Nucl N-term GFP

Plasmid

#36123

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human, contains CypB AA 33-216)

Use

Tags

GFP

Expression

Mammalian

Mutation

A212S

Promoter

CMV

Available since

Nov. 1, 2012

Availability

Academic Institutions and Nonprofits only
p3 FLAG_CMV14/OCTN2 (wt)

Plasmid

#112799

Purpose

Expression of OCTN2

Depositor

Katarzyna Nalęcz

Article

Czeredys et al PLoS One. 2013 Dec 13;8(12):e82105. doi:

Insert

OCTN2/SLC22A5 (Slc22a5 Rat)

Use

Tags

3 x Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTPTP alpha (S204A)

Plasmid

#17694

Depositor

Xinmin Zheng

Article

Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

Insert

PTPalpha S204A (PTPRA Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S204A

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
L1-1151Y>A

Plasmid

#13269

Depositor

Vance Lemmon

Article

Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

Insert

L1CAM (L1CAM Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pUWR-Cad-mTFP

Plasmid

#58327

Purpose

Destination vector for inserting ubi-Cad-mTFP to Drosophila melanogaster genome

Depositor

Denise Montell

Article

Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

Insert

Cad-mTFP

Use

Tags

Expression

Insect

Mutation

Promoter

poly-ubiquitin

Available since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-myc-CD4-Sema4D

Plasmid

#51603

Purpose

expresses Sema4D/CD4 chimera with intracellular Sema4D, extracellular CD4

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

CD4-Sema4D

Use

Tags

myc

Expression

Mammalian

Mutation

Sema4D amino acids 1-754 have been replaced with …

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pCDF1-ERBB4 (E452K)

Plasmid

#29538

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
GST-AR-4-52

Plasmid

#104199

Purpose

Expresses human AR-4-52 in in pGEX-4T-1 as GST fusion protein

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

human androgen receptor aa 4-52 (AR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKP4010

Plasmid

#113336

Purpose

Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Inserts

Artificial Transcription Factor (McIsaac et al, 2013)
Ty3 Integrase

Use

Tags

3X-FLAG/8X-Gly linker

Expression

Yeast

Mutation

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-intron-BaLgp160

Plasmid

#100918

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env with upstream intron

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS_AKAR5’

Plasmid

#114141

Purpose

Mammalian expression of the AKAR5’ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

AKAR5'

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB-Flag-MyoD-E12

Plasmid

#25990

Depositor

Jeffrey Dilworth

Article

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD-E12 Heterodimer Fusion (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Expression

Mutation

Promoter

Available since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge