Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 7801 - 7820 of 13964 results

  3. pAAV-MP-84-spCas9-U6-gRNA-tlock

    Plasmid
    #206936
    Purpose
    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.
    Depositor
    Markus Grompe
    Insert
    spCas9
    Use
    AAV
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    MP-84
    Availability
    Academic Institutions and Nonprofits only
    206936-plasmid-map-sequence-id-407723

    Enlarge

  4. 5J39 (TDRKH)

    Plasmid
    #79523
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TDRD2 (TDRKH Human)
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Academic Institutions and Nonprofits only
    79523-plasmid-map-sequence-id-150161

    Enlarge

  5. 5J3E (THYN1)

    Plasmid
    #79524
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    THYN1 (THYN1 Human)
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Academic Institutions and Nonprofits only
    79524-plasmid-map-sequence-id-150164

    Enlarge

  6. pVP16-tev-PRT1-V28A

    Plasmid
    #83406
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    V28A substitution
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    83406-plasmid-map-sequence-id-175082

    Enlarge

  7. pVP16-tev-PRT1-C29A

    Plasmid
    #83407
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    C29A substitution
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    83407-plasmid-map-sequence-id-175083

    Enlarge

  8. pVP16-tev-PRT1-C195A

    Plasmid
    #83408
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    C195A substitution
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    83408-plasmid-map-sequence-id-175084

    Enlarge

  9. pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro

    Plasmid
    #50417
    Purpose
    Fluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.
    Depositor
    Andrew Scharenberg
    Inserts
    eGFP with I-Sce I TS
    +3 mCherry
    Use
    Lentiviral
    Tags
    T2A dislinker and iRFP
    Expression
    Mammalian
    Mutation
    embedded I-Sce I TS from 163-185
    Promoter
    SFFV
    Availability
    Academic Institutions and Nonprofits only
    50417-plasmid-map-sequence-id-75349

    Enlarge

  10. pCVL RipTAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers

    Plasmid
    #50626
    Purpose
    pRipTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    50626-plasmid-map-sequence-id-79595

    Enlarge

  11. AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato

    Plasmid
    #51082
    Purpose
    Cre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV1
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1a
    Availability
    Academic Institutions and Nonprofits only
    51082-plasmid-map-sequence-id-175187

    Enlarge

  12. AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51083
    Purpose
    Cre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV Retrograde
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1a
    Availability
    Academic Institutions and Nonprofits only
    51083-plasmid-map-sequence-id-175189

    Enlarge

  13. AAV-CaMKIIa-GCaMP6s-P2A-nls-dTomato

    Plasmid
    #51086
    Purpose
    Forebrain principle neuron specific expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa 1.3 kb
    Availability
    Academic Institutions and Nonprofits only
    51086-plasmid-map-sequence-id-77368

    Enlarge

  14. AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51087
    Purpose
    Forebrain principle neuron specific expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa 1.3 kb
    Availability
    Academic Institutions and Nonprofits only
    51087-plasmid-map-sequence-id-77369

    Enlarge

  15. BC-GPRC

    Plasmid
    #44199
    Depositor
    Lei Xu
    Insert
    human GPR56 C-terminal fragment (ADGRG1 Human)
    Use
    Retroviral
    Tags
    none
    Expression
    Mammalian
    Mutation
    C-terminal fragment of GPR56 starting at amino a…
    Promoter
    LTR
    Availability
    Academic Institutions and Nonprofits only
    44199-plasmid-map-sequence-id-60058

    Enlarge

  16. UBC64

    Plasmid
    #25143
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubc64.248.414 (UBE2Q1 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    contains UBC domain; residues 248-414
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. NR1D2

    Plasmid
    #25186
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NR1D2_07 (NR1D2 Human)
    Use
    Baculovirus expression
    Tags
    His
    Expression
    Mutation
    Promoter
    polyhedrin
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. NFATC2IP

    Plasmid
    #25221
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubh72.342.419 (NFATC2IP Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. UHRF1

    Plasmid
    #25359
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubh12.672.793 (UHRF1 Human)
    Use
    Tags
    His with thrombin cleavage site
    Expression
    Bacterial
    Mutation
    amino acid residues 672-793.
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. NR4A1

    Plasmid
    #25477
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NR4A1 (NR4A1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 7801 - 7820 of 13964 results