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Plasmid#192654Purpose3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance markerDepositorInsertVP64-dCas9-p300
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A; H840A in Cas9PromoterEF1aAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LSL-Cas9-Rosa26TV
Plasmid#61408PurposeCre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.DepositorInsertsCas9
EGFP
UseCRISPR, Cre/Lox, and Mouse TargetingTags3xFLAG and P2AExpressionMammalianMutationhuman codon optimizedPromoterCAGAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLSC-5
Plasmid#62889PurposeLenti vector for expression of inducible split Cas9DepositorInsertsSpCas9(574-1368)
SpCas9(2-573)
UseCRISPR and LentiviralTagsFFBP12, FRB, NES PTK2, NLS SV40, and P2AExpressionMammalianMutation*see comment belowPromoterEFSAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMLS485
Plasmid#188890PurposeWorm Cas9 expression vector, Peft-3 promoterDepositorInsertPeft-3::Cas9
UseTagsExpressionWormMutationC. elegans codom optimization and intron additionPromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAW212
Plasmid#113234PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->CCATCDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->CCATCPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRS-GFP
Plasmid#220197Purposemini-Tn7 GFP expression vectorDepositorInsertmonomeric superfolder green fluorescent protein
UseCRISPRTagsExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpromoterlessAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAW228
Plasmid#113242PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader tAggtag mutantDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationTTGGTAG->tAggtagPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAW227
Plasmid#113241PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Atggtag mutantDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationTTGGTAG->AtggtagPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAW218
Plasmid#113240PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flipDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationAAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…PromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW217
Plasmid#113239PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtaC mutantDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->ggtaCPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW215
Plasmid#113237PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggAag mutantDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->ggAagPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW214
Plasmid#113236PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader gCtag mutantDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->gCtagPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW213
Plasmid#113235PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader Cgtag mutantDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->CgtagPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW211
Plasmid#113233PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->AACGADepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->AACGAPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW216
Plasmid#113238PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader ggtTg mutantDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->ggtTgPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W
Plasmid#72666PurposeLentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)DepositorInserth7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W
Plasmid#72667PurposeLentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)DepositorInsertmU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(BsmBI)-EF1a-BFP-BSD
Plasmid#117136PurposeEmpty vector encoding BFP-BSDDepositorInsertBSD2A
UseLentiviralTagsExpressionMammalianMutationmodified BFPPromoterhuman U6 promoter, human EF1a promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
IL1RN gRNA1
Plasmid#113130PurposeExpresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)DepositorInsertIL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6 for gRNA expression, RSV for GFP expressionAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMamCol1
Plasmid#215184PurposeAllows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coliDepositorInserttsPurple
UseAAV, CRISPR, and Synthetic Biology ; Recombinant …TagsSNAC-StrepII tagExpressionBacterial and MammalianMutationPromoterTetON 3G Bidirectional for Mammalian and Trc with…Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only