Skip to main content
Addgene
Showing: 1901 - 1950 of 15602 results

  7. pACEC3GH (Hyg)

    Plasmid
    #110084
    Purpose
    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    3C cleavage site - GFP - His10 Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110084-plasmid-map-sequence-id-211189

    Enlarge

  10. pFLAG_attP

    Plasmid
    #110095
    Purpose
    Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110095-plasmid-map-sequence-id-317064

    Enlarge

  12. pACEC3GH (Apr)

    Plasmid
    #110085
    Purpose
    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    3C cleavage site - GFP - His10 Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110085-plasmid-map-sequence-id-211188

    Enlarge

  17. pCHC005 (∆RE)

    Plasmid
    #226199
    Purpose
    Conjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).
    Depositor
    Christopher Johnson
    Article
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Homology arms for type I restriction-modification system restriction subunit H16_A0006
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226199-plasmid-map-sequence-id-453519

    Enlarge

  20. PtetA-PLacO3O1 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid
    #225645
    Purpose
    tetA and LacO3O1 promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance
    Depositor
    Andre Sanches Ribeiro
    Article
    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/
    Insert
    tetR/tetA and LacO3O1 in tandem conformation
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    tetA and LacO3O1 promoters
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    225645-plasmid-map-sequence-id-448703

    Enlarge

  21. PLacO3O1-PtetA 250 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid
    #225648
    Purpose
    LacO3O1 and tetA promoters in tandem conformation with 250bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance
    Depositor
    Andre Sanches Ribeiro
    Article
    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/
    Insert
    LacO3O1 and tetR/tetA in tandem conformation
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    LacO3O1 and tetA promoters
    Available since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    225648-plasmid-map-sequence-id-448715

    Enlarge

  22. PLacO3O1-PtetA 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid
    #225647
    Purpose
    LacO3O1 and tetA promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance
    Depositor
    Andre Sanches Ribeiro
    Article
    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/
    Insert
    LacO3O1 and tetR/tetA in tandem conformation
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    LacO3O1 and tetA promoters
    Available since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    225647-plasmid-map-sequence-id-448712

    Enlarge

  23. PtetA-PLacO3O1 350 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid
    #225646
    Purpose
    tetA and LacO3O1 promoters in tandem conformation with 350bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance
    Depositor
    Andre Sanches Ribeiro
    Article
    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/
    Insert
    tetR/tetA and LacO3O1 in tandem conformation
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    tetA and LacO3O1 promoters
    Available since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    225646-plasmid-map-sequence-id-448711

    Enlarge

  34. pET-Ce25

    Plasmid
    #51296
    Purpose
    Expresses a plant arginyl-tRNA synthetase in E.coli
    Depositor
    Gabor Igloi
    Article
    Hogg et al Protein Expr Purif. 2008 Oct;61(2):163-7
    Insert
    arginyl-tRNA synthetase
    Use
    Tags
    His Tag and Thioredoxin
    Expression
    Bacterial
    Mutation
    GTT (Val) insertion after initiation codon to per…
    Promoter
    T7
    Available since
    July 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    51296-plasmid-map-sequence-id-78029

    Enlarge

  36. pBHM2403

    Plasmid
    #171687
    Purpose
    pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.
    Depositor
    Hiten Madhani
    Article
    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi
    Insert
    CnoCAS9
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    TEF1
    Available since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    171687-plasmid-map-sequence-id-340174

    Enlarge

  38. pCHC023 (∆MBH)

    Plasmid
    #226201
    Purpose
    Electroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).
    Depositor
    Christopher Johnson
    Article
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Homology arms for membrane bound hydrogenase operon
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    ∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJ
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226201-plasmid-map-sequence-id-453504

    Enlarge

  41. pCHC027 (∆pemK)

    Plasmid
    #226204
    Purpose
    Conjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.
    Depositor
    Christopher Johnson
    Article
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Upstream/downstream homology arms for addiction system toxinPemK.
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226204-plasmid-map-sequence-id-452438

    Enlarge

  42. PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid
    #225649
    Purpose
    tetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance
    Depositor
    Andre Sanches Ribeiro
    Article
    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/
    Insert
    tetR/tetA, araBAD and LacO3O1 in tandem conformation
    Use
    Tags
    mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    tetA, araBAD and LacO3O1 promoters
    Available since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    225649-plasmid-map-sequence-id-448716

    Enlarge

  48. pDD2000

    Plasmid
    #101197
    Purpose
    Name: pBluescript-LANApi,K14. Bidirectional reporter driving LANApi side [green luciferase isoform from pCBG68] and K14 side [red luciferase isoform from pCBR] in pBluescript backbone.
    Depositor
    Dirk Dittmer
    Article
    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1
    Insert
    LANApi side [green luciferase isoform of pCBG68], K14 side [red luciferase isoform of pCBR]
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    101197-plasmid-map-sequence-id-194526

    Enlarge

Showing: 1901 - 1950 of 15602 results