pESUMO-hRORg2
(Plasmid
#170329)
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PurposeExpresses RORg isoform 2 with N-terminal HisSUMO tag in bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 170329 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepESUMO
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Backbone manufacturerLifeSensor
- Backbone size w/o insert (bp) 5476
- Total vector size (bp) 7236
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRORG isoform 2
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Alt nameRORgammaT
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SpeciesH. sapiens (human)
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Insert Size (bp)1501
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Mutationcodon optimized
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Entrez GeneRORC (a.k.a. IMD42, NR1F3, RORG, RZR-GAMMA, RZRG, TOR)
- Promoter T7
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Tag
/ Fusion Protein
- HisSUMO (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer ACCACTCCTTTAAGAAGGC
- 3′ sequencing primer GCTAGTTATTGCTCAGCGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pESUMO-hRORg2 was a gift from Patrick Griffin (Addgene plasmid # 170329 ; http://n2t.net/addgene:170329 ; RRID:Addgene_170329) -
For your References section:
Conformational Changes of RORgamma During Response Element Recognition and Coregulator Engagement. Strutzenberg TS, Zhu Y, Novick SJ, Garcia-Ordonez RD, Doebelin C, He Y, Chang MR, Kamenecka TM, Edwards DP, Griffin PR. J Mol Biol. 2021 Sep 20;433(22):167258. doi: 10.1016/j.jmb.2021.167258. 10.1016/j.jmb.2021.167258 PubMed 34547329