pEGFP-mAV
(Plasmid #129472)

• Purpose: Mammalian expression of full-length mouse archvillin (Supervillin, isoform 2)
• Depositing Lab: Elizabeth Luna
• Publication: Oh et al J Cell Sci. 2003 Jun 1. 116(Pt 11):2261-75.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4731
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Supervillin, isoform 2
• Alt name: Archvillin
• Alt name: Svil
• Alt name: mSV2
• Species: M. musculus (mouse)
• Entrez Gene: Svil (a.k.a. AU024053, B430302E16Rik)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: SacII (unknown if destroyed)
• 5′ sequencing primer: EGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry