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Plasmid#196864PurposeExpression of neural precursor cell expressed developmentally down-regulated 1 (Need1) fused to mNeonGreenDepositorInsertNedd1-mNeonGreen (Nedd1 Rat)
UseTagsExpressionMammalianMutationPromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
Polymerase expression - pCMV-ePhi29 (LM2802)
Plasmid#208967PurposeUnfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.DepositorInsertePhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…PromoterCMV and T7Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-mCherry-KATNA1
Plasmid#180650PurposeLentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47.DepositorInsertKATNA1 (KATNA1 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationsiRNA resistant to: GGACAGCACUCCCUUGAAAPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-actin-R62D
Plasmid#118379PurposeGateway compatible donor vector with actin-R62D.DepositorInsertactin beta (ACTB Human)
UseDonor-plasmidTagsExpressionMutationR62DPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM50
Plasmid#123656PurposeEncodes Renilla luciferase under the Aedes aegypti ubiquitin UbL40 promoter for constitutive expression in mosquito cellsDepositorInsertAedes aegypti UbL40 promoter
UseLuciferaseTagsExpressionInsectMutationPromoterUbL40 promoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cdt1 human
Plasmid#72681Purposemammalian expression of human Cdt1DepositorInsertCdt1 (CDT1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-ArchT-GFP
Plasmid#58851PurposeAAV-mediated expression of ArchT-GFP under the EF1a promoter, in floxed/reversed (Cre-dependent) mannerDepositorInsertArchT-GFP
UseAAV and Cre/LoxTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
CaMKIV-DCTK75E
Plasmid#126423PurposeA kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)DepositorInsertcalcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
UseTagsFLAGExpressionMammalianMutationchanged lysine 75 to glutamic acidPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS3/myc-His
Plasmid#127487PurposeExpresses myc-tagged NDUFS3 in mammalian cellsDepositorInsertNDUFS3 (NDUFS3 Human)
UseTagsmyc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFS2/myc-His
Plasmid#127486PurposeExpresses myc-tagged NDUFS2 in mammalian cellsDepositorInsertNDUFS2 (NDUFS2 Human)
UseTagsmyc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARMA2sh-HA
Plasmid#97072PurposeExpresses CARMA2sh/CARD14 isoform 3 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
UseTagsHAExpressionMammalianMutationnonePromoterCMVAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C1-Eps8 WT
Plasmid#74950Purposemammalian expression of Eps8 fused to GFPDepositorInsertEps8 (Eps8 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTEF:ATP
Plasmid#92179PurposeHO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.DepositorInsertATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
UseIntegration and expression into yeast.TagsYeast codon optimized mseCFP (donor) and cp173-mV…ExpressionYeastMutationPromoterTEF1pAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mouse Alpha2 delta-2 HA pMT2
Plasmid#58731Purposeexpression of HA tagged mouse Alpha2 delta-2 in mamalian cellsDepositorInsertalpha2 delta-2 (Cacna2d2 Mouse)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/NDUFV1/myc-His
Plasmid#127490PurposeExpresses myc-tagged NDUFV1 in mammalian cellsDepositorInsertNDUFV1 (NDUFV1 Human)
UseTagsmyc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKM19
Plasmid#123655PurposeEncodes firefly luciferase under the pIEx promoter for constitutive expression in insect (e.g. Drosophila, mosquito) cellsDepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionInsectMutationPromoterpIExAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONOR223 R2pH-LAMP1-3xFLAG
Plasmid#157941Purposegateway donor vector encoding ratiometric sensor of lysosomal lumenal pHDepositorInsertR2pH-LAMP1-3xFLAG (Lamp1 Mouse)
UseGateway systemTags3XFLAG, mCherry, and pHluorinExpressionMutationPromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHIV-ARF-zsGreen
Plasmid#110729PurposeMammalian Expression of p14ARF IRES zsGreenDepositorInsertp14ARF IRES zsGreen (CDKN2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gal4-entry+NLS
Plasmid#128013PurposeVector containing the Gal4-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertGal4 DBD-SV40NLS-3x-Flag
UseGal4 entry vector with nls to generate tethering …TagsGal4-DBD-SV40NLS-3xFlagExpressionMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-FH-Nsp13_GC3opt
Plasmid#157715Purposemammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp13_GC3opt (ORF1ab SARS-CoV-2)
UseTagsFlag-His8ExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits