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Addgene
Showing: 7601 - 7620 of 13964 results

  1. 5C7J

    Plasmid
    #69965
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NEDD4:SDC094-F02:C29544 (NEDD4 Human)
    Use
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Academic Institutions and Nonprofits only

  2. 5C8H

    Plasmid
    #69969
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ORC2:JMC095-D11:C231601 (ORC2 Human)
    Use
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Academic Institutions and Nonprofits only

  3. pFHMSP-LIC-N

    Plasmid
    #26095
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    His and TEV cleavage site
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. PP-MAPK1 (3OZ6)

    Plasmid
    #28164
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    mitogen-activated protein kinase 1 (cgd2_1960 Cryptosporidium parvum)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains residues 4-390
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. MUM1

    Plasmid
    #28273
    Depositor
    Cheryl Arrowsmith
    Insert
    MUM1 (MUM1 Human)
    Use
    Tags
    His with TEV cleavage
    Expression
    Bacterial
    Mutation
    Contains PWWP Domain (residues 405-538).
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. TDRD3 (3PMT)

    Plasmid
    #28274
    Depositor
    Cheryl Arrowsmith
    Insert
    Tudor domain-containing protein 3 (TDRD3 Human)
    Use
    Tags
    His with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains Tudor domain, residues 553-611.
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. PP-CDPK1

    Plasmid
    #25280
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    cgd5_820:D42-E517 (cgd5_820 Cryptosporidium parvum)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    Contains amino acids D42-E517.
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. pCMV-P10HA

    Plasmid
    #1345
    Depositor
    Li-Huei Tsai
    Insert
    P10 (CDK5R1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  10. pHL1947

    Plasmid
    #53045
    Purpose
    PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHindM8:RBS(st3):csrA
    Depositor
    Han Lim
    Inserts
    GFP
    tetR
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st3) and glgC leader
    Expression
    Bacterial
    Mutation
    Promoter
    pConNoHind, pConNoHindM12, and pConNoHindM8
    Availability
    Academic Institutions and Nonprofits only

  11. pHL1948

    Plasmid
    #53046
    Purpose
    PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHindM10:RBS(st3):csrA
    Depositor
    Han Lim
    Inserts
    GFP
    tetR
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st3) and glgC leader
    Expression
    Bacterial
    Mutation
    Promoter
    pConNoHind, pConNoHindM10, and pConNoHindM12
    Availability
    Academic Institutions and Nonprofits only

  12. 5CZG (Tb427.10.7420)

    Plasmid
    #79517
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Tb427.10.7420
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Academic Institutions and Nonprofits only
    79517-plasmid-map-sequence-id-150098

    Enlarge

  13. 5FEA (LdBRD19, LdBPK_363520.1)

    Plasmid
    #79520
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    LdBPK_363520.1
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Academic Institutions and Nonprofits only
    79520-plasmid-map-sequence-id-150104

    Enlarge

  14. 5J3U (TgPKA, 583.m05422)

    Plasmid
    #79525
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    583.m05422
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Academic Institutions and Nonprofits only
    79525-plasmid-map-sequence-id-150111

    Enlarge

  15. pMSP1D1Q1

    Plasmid
    #97326
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional Q residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Insert
    MSP1D1Q1
    Use
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of Q residue after T25
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    97326-plasmid-map-sequence-id-182622

    Enlarge

  16. NUDT7 (NUDT7A-c002)

    Plasmid
    #98235
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT7A (NUDT7 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    98235-plasmid-map-sequence-id-193415

    Enlarge

  17. FAM83B (FAM83BA-c004)

    Plasmid
    #98236
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FAM83BA
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    98236-plasmid-map-sequence-id-227301

    Enlarge

  18. EBFP2-PCNA-19-SV40NLS-4

    Plasmid
    #55250
    Purpose
    Localization: Nucleus, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Use
    Tags
    3xSV40 NLS and EBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    55250-plasmid-map-sequence-id-230171

    Enlarge

  19. Dendra2-PCNA-19-SV40NLS-4

    Plasmid
    #57735
    Purpose
    Localization: Nucleus, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Use
    Tags
    Dendra2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pSaCas9_GFP

    Plasmid
    #64709
    Purpose
    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
    Depositor
    Kiran Musunuru
    Insert
    SaCas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP and 3xFLAG-SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    64709-plasmid-map-sequence-id-322473

    Enlarge

Showing: 7601 - 7620 of 13964 results