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Plasmid#132587PurposegRNA used for knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele (MYH11-NanoLuc-2A-tdTomato vector) )DepositorInsertMYH11-gRNA1
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDragon-Ctgf
Plasmid#155015PurposeFor Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to connective tissue growth factor in the presence of Cre and (r)tTA activity.DepositorInsertCcn2 (Ccn2 Mouse)
UseMouse TargetingTagsExpressionMutationM24T (please see depositor comments)PromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV pCAG-mRuby3-WPRE
Plasmid#107744PurposeCan be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.DepositorInsertmRuby3
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFPnls barcode-206-SV40 polyA
Plasmid#190875PurposeFor barcoded retrograde labelingDepositorInsertEGFPnls-barcode206
UseAAVTagsExpressionMutationWTPromoterAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
UPB-esophagus (UPB5)
Plasmid#180783PurposeUnique Projection Barcode (UPB5)-Esophagus for Projection-seqDepositorInserthM3Dq ( partial ) (CHRM3 Human)
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-CIBN
Plasmid#183937PurposePCP tandem dimer binding to PP7 RNA stem loops coupled to optogenetic CIBN domain; bound by PHR upon blue light exposureDepositorInserttdPCP-CIBN (CIB1 Synthetic, Mustard Weed)
UseTagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-rBrokenheart
Plasmid#203394PurposeAAV transfer plasmid containing a constitutive (non Cre-dependent) BrokenHeart construct: a piggyBac donor transposon interrupting tdTomato. Transposase activity rescues coding sequence.DepositorInsertBrokenheart
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP barcode-10-SV40 polyA
Plasmid#190874PurposeFor barcoded retrograde labelingDepositorInsertEGFP-barcode10
UseAAVTagsExpressionMutationWTPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMADM-alpha
Plasmid#36890DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
Neo
ATG (T)
GFP
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, deleted…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA
Plasmid#177360PurposeAAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoterDepositorInsertstdTomato
Chimeric guide for SaCas9
UseAAVTagsP2A and a tetracycline-controlled transactivator …ExpressionMammalianMutationPromoterSynapsine promoter and U6 promoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA
Plasmid#177365PurposeAAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expressionDepositorInsertstdTomato
Chimeric guide for SaCas9
UseAAVTagsP2A and a tetracycline-controlled transactivator …ExpressionMammalianMutationPromoterCMV and U6 promoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2705-AGER-eGFP
Plasmid#216474Purposedonor vector for targeting an eGFP reporter to the human AGER locus at the endogenous ATG start siteDepositorInserteGFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
B2M-SDMutation-EhT
Plasmid#215546PurposeDonor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-outDepositorInsertB2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
UseCRISPRTagsExpressionMutationThe second base of the first intron of B2M (From …PromoterAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFPnls barcode-210-SV40 polyA
Plasmid#190876PurposeFor barcoded retrograde labelingDepositorInsertEGFPnls-barcode210
UseAAVTagsExpressionMutationWTPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP barcode-9-SV40 polyA
Plasmid#190873PurposeFor barcoded retrograde labelingDepositorInsertEGFP-barcode9
UseAAVTagsExpressionMutationWTPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD
Plasmid#199447PurposeExpession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signalDepositorInsertdSaCas9-10xGCN4
UseLentiviralTagsExpressionMammalianMutationPromoterhuman ubiquitin C (UBC)Available sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP barcode-7-SV40 polyA
Plasmid#190871PurposeFor barcoded retrograde labelingDepositorInsertEGFP-barcode7
UseAAVTagsExpressionMutationWTPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP barcode-8-SV40 polyA
Plasmid#190872PurposeFor barcoded retrograde labelingDepositorInsertEGFP-barcode8
UseAAVTagsExpressionMutationWTPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-EGFP barcode-6-SV40 polyA
Plasmid#190870PurposeFor barcoded retrograde labelingDepositorInsertEGFP-barcode6
UseAAVTagsExpressionMutationWTPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNOC_dCas9_sgRNA Td2
Plasmid#176258PurposepNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2DepositorInsertdead spCas9
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationH840A and D10A mutations on spCas9 to inactivate …PromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only