Skip to main content
Addgene
Showing: 1851 - 1900 of 15599 results

  5. pJK599

    Plasmid
    #72524
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72524-plasmid-map-sequence-id-132609

    Enlarge

  6. pJK618

    Plasmid
    #72535
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His6 tag; glutaminase subunit; large subunit, C-terminal His6 tag (H6AaPurS, AaPurQ, AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72535-plasmid-map-sequence-id-132626

    Enlarge

  7. pZY101

    Plasmid
    #73932
    Purpose
    This binary vector, also named PTF101, is used as a standard plant transformation vector. For more information, please see http://plantsci.missouri.edu/muptcf/pzy101.html
    Depositor
    Zhanyuan Zhang
    Article
    Zeng et al Plant Cell Rep. 2004 Feb;22(7):478-82. E
    Insert
    bar
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    73932-plasmid-map-sequence-id-139928

    Enlarge

  10. pKO (Kan)

    Plasmid
    #110086
    Purpose
    Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Bacterial knockout generation
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    110086-plasmid-map-sequence-id-264226

    Enlarge

  11. pKO (Apr)

    Plasmid
    #110088
    Purpose
    Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Bacterial knockout generation
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110088-plasmid-map-sequence-id-211180

    Enlarge

  12. pTR47m4

    Plasmid
    #104068
    Purpose
    Luciferase Reporter for Formaldehyde Sensing
    Depositor
    David Liu
    Article
    Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.
    Inserts
    xluxAB
    frmR
    frm Promoter m4
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    23r and 25th nucleotides in sequence mutated from…
    Promoter
    Available since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    104068-plasmid-map-sequence-id-205132

    Enlarge

  13. pKO (Hyg)

    Plasmid
    #110087
    Purpose
    Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Bacterial knockout generation
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110087-plasmid-map-sequence-id-316821

    Enlarge

  15. pMINTNH3

    Plasmid
    #110074
    Purpose
    Integrative vector for expression in mycobacteria based on the Tet promoter. Contains an N-terminal His10 Tag followed by a 3C cleavage site
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    His10 Tag - 3C cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110074-plasmid-map-sequence-id-264216

    Enlarge

  31. pJK571

    Plasmid
    #75379
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits and associated hypothetical protein with N-terminal His6 tag (AaPurS, H6AaOrfY, AaPurQ, AaPurL-G209D)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Gene synthesis
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glycine-209 to aspartate and synthetic ge…
    Promoter
    n/a
    Available since
    Aug. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    75379-plasmid-map-sequence-id-142756

    Enlarge

  34. pJK598

    Plasmid
    #72523
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with C-terminal His6 tag, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72523-plasmid-map-sequence-id-132608

    Enlarge

  35. pJK612

    Plasmid
    #72533
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    residues 1-69 only; C-terminal domain removed and…
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72533-plasmid-map-sequence-id-132621

    Enlarge

  36. pJK613

    Plasmid
    #72534
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with His6 tag; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    residues 1-69 only; C-terminal domain removed and…
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72534-plasmid-map-sequence-id-132623

    Enlarge

  37. pJK619

    Plasmid
    #72536
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His-tag; hypothet. protein; glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72536-plasmid-map-sequence-id-132627

    Enlarge

  40. GFP-V96 ELP

    Plasmid
    #67853
    Purpose
    GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues
    Depositor
    Andrew MacKay
    Article
    Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34
    Insert
    GFP fused to ELP (VPGVG) repeated 96 times
    Use
    Tags
    GFP fused to N-terminal of ELP
    Expression
    Mammalian
    Mutation
    Promoter
    T7
    Available since
    Oct. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    67853-plasmid-map-sequence-id-117808

    Enlarge

  49. pPEE17

    Plasmid
    #128361
    Purpose
    Detecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Insert
    Acetamidase (amdS)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    TEF1
    Available since
    Jan. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    128361-plasmid-map-sequence-id-267942

    Enlarge

Showing: 1851 - 1900 of 15599 results