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Addgene

pBSFI-p110δ
(Plasmid #34893)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 34893 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBSFI
  • Backbone manufacturer
    Aoki et al., PNAS. 1998 Dec 8;95(25):14950-5
  • Backbone size w/o insert (bp) 2949
  • Vector type
    Adaptor plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p110δ
  • Alt name
    PIK3CD
  • Species
    H. sapiens (human)
  • Mutation
    See depositor comments below.
  • Entrez Gene
    PIK3CD (a.k.a. APDS, IMD14, IMD14A, IMD14B, P110DELTA, PI3K, ROCHIS, p110D)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS results found a P2A variant compared to the NCBI reference [NP_005017.3].

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBSFI-p110δ was a gift from Peter Vogt (Addgene plasmid # 34893 ; http://n2t.net/addgene:34893 ; RRID:Addgene_34893)

  • For your References section:

    Oncogenic transformation induced by the p110beta, -gamma, and -delta isoforms of class I phosphoinositide 3-kinase. Kang S, Denley A, Vanhaesebroeck B, Vogt PK. Proc Natl Acad Sci U S A. 2006 Jan 31;103(5):1289-94. Epub 2006 Jan 23. 10.1073/pnas.0510772103 PubMed 16432180
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