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pHAGE2-TetOminiCMV-SKM

Plasmid

#136551

Purpose

Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Sox2, Klf4, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Sox2-T2A-Klf4-E2A-cMyc (Sox2, Klf4, Myc Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-Enh eGFP Reporter-huKC2-IGR21

Plasmid

#59456

Purpose

Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the human keratin type II cluster.

Depositor

Benjamin Yu

Insert

huKrt1 3’intergenic region

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Mu Hsp68 minimal promoter

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti

Plasmid

#115795

Purpose

Expresses AcrIIA4 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrIIA4

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

EF-1a promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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TFORF2972

Plasmid

#144448

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NR1H4 (NR1H4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-SM

Plasmid

#136578

Purpose

Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, cMyc

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Sox2-E2A-cMyc (Sox2, Myc Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLX_313-EGFP

Plasmid

#158580

Purpose

Lentiviral expression of EGFP

Depositor

David Root, John Doench

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-AcGFP-P2A-hBRD4S

Plasmid

#197882

Purpose

study roles of human BET proteins in human pluripotency reprogramming

Depositor

Kejin Hu

Article

Hossain et al iScience. 2022 Dec 28;26(1):105889. doi:

Insert

BRD4 (BRD4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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EFS eGFP hPGK BSD

Plasmid

#188520

Purpose

Backbone for CRISPR interference (CRISPRi) isogenic PAM testing

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Type

Empty backbone

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Baf60c

Plasmid

#72691

Purpose

Doxycyline regulated expression of Baf60c in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Brg 1 (SMARCD3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CB-E470K

Plasmid

#116554

Purpose

Lentiviral expression of PIK3CB E470K

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CB (PIK3CB Human)

Use

Lentiviral

Tags

Expression

Mutation

E470K

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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ERBB2 gRNA (BRDN0001146057)

Plasmid

#76376

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERBB2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERBB2 (ERBB2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TFORF1682

Plasmid

#142825

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SIM1 (SIM1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF0696

Plasmid

#141665

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

GTF3A (GTF3A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAH1 (lenti hLPL-FLAG)

Plasmid

#102448

Purpose

FLAG-tagged human LPL in lentiviral vector

Depositor

Brandon Davies

Article

Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

Insert

Lipoprotein lipase (LPL Human)

Use

Lentiviral

Tags

6x His and FLAG

Expression

Mutation

Promoter

CMV

Available since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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CDK12 gRNA (BRDN0001147156)

Plasmid

#75915

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK12

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK12 (CDK12 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1 RagC shRNA #1

Plasmid

#26629

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagC shRNA (RRAGC Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pcsII-pb6-flag

Plasmid

#86770

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

proteasome beta subunit 6 (PSMB6 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

Promoter

unknown

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Gata3

Plasmid

#128824

Purpose

dox-dependent expression of mouse Gata3

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Gata3 (Gata3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W

Plasmid

#163169

Purpose

Lentiviral plasmid with non-targeting gRNA, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

Non-targeting

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-hU6-mU6-BlasGO

Plasmid

#136906

Purpose

Lentivirus for base editing activatable Blasticidin resistance gene expression in mammalian cells. All in one vector with sgBlasGO.

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

BlasCyGO

Use

Lentiviral

Tags

Expression

Mutation

ATG>ACG

Promoter

SFFV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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