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Plasmid#185680PurposeLow-level transient expression of full-length HsIRE1 with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hAtg12
Plasmid#87869PurposeExpress a EGFP-human Atg12/Apg12 in mammalian cellsDepositorInsertATG12 (ATG12 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-deSpCas9
Plasmid#92114PurposeExpression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)DepositorInsertdead/inactive eSpCas9 with FLAG tag
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationD10A, H840A, K848A, K1003A, R1060APromoterCbhAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX304_zeo
Plasmid#160092PurposeLentiviral vector for mammalian gene expressionDepositorTypeEmpty backboneUseLentiviral; Gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nrp2.1-Fc-His
Plasmid#72098PurposeExpresses the extracellular region of the Neuropilin 2, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertNrp2.1 (Nrp2 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
SID4x-dCas9-KRAB
Plasmid#106399PurposeExpresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domainDepositorUseTagsFLAG, HA, and NLSExpressionMammalianMutationPromoterAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
TrHKI-pGFPN3
Plasmid#21918DepositorInsertTruncated human HKI (lacking exon 1 sequence that encodes for the mitochondrial binding domain) in pGFP-N3 plasmid (HK1 Human)
UseTagsGFPExpressionMammalianMutationThis is the truncated human HKI cDNA (lacking exo…PromoterAvailable sinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pZW16 (CK2alpha')
Plasmid#27087DepositorInsertCK2alpha' (CSNK2A2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
BMR1_01g02031-bio-His
Plasmid#108116Purpose[Edit] Expresses enzymatically monobiotinylated full-length BMR1_01g02031 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tagDepositorInsertBMR1_01g02031
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Myc-mMEKK2
Plasmid#44158DepositorInsertMekk2 (Map3k2 Mouse)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAL119-Flt3L
Plasmid#21910DepositorInsertHuman Soluble Fms-like Tyrosine Kinase 3 Ligand (FLT3LG Human)
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pAuroraA-GFP-AURKA_K162M-mCherry
Plasmid#99880PurposeExpression of GFP-AURKA K162M-mCherry in cellsDepositorInsertAURKA (AURKA Human)
UseTagsGFP and mCherryExpressionMammalianMutationK162M (kinase-dead)PromoterAURKA minimalAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
ABE8.17-m
Plasmid#136298Purposeexpresses ABE8.17-m in mammalian cellsDepositorInsertABE8.17-m
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pTT3 Unc5A-FLAG
Plasmid#72194PurposeExpresses full-length Unc5a with a C-terminal FLAG tagDepositorInsertUnc5a (Unc5a Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)
Plasmid#140892PurposeExpresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.DepositorUseTagsmCherryExpressionMammalianMutationchanged 223L to F in Dr-VSP (zebrafish TPTE)PromoterAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-mTagBFP2-Blasticidin
Plasmid#167207PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with BlasticidinDepositorTypeEmpty backboneUseCRISPRTagsmTagBFP2ExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-FlpOn-CreOff-MCS-P2A-mCherry
Plasmid#176277PurposeViral vector for co-expression of a CDS of interest and mCherry in cells expressing Flp AND NOT Cre driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.DepositorInsertMCS-P2A-mCherry
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman Synapsin IAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-HA-MEK5
Plasmid#53166PurposeExpresses GST-HA tagged human MEK5 from bacterial expression vectorDepositorInsertMEK5 (MAP2K5 Human)
UseTagsGST and HAExpressionBacterialMutationPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only