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Plasmid#136685PurposeExpresses the Beclin-1 ECD/BARA domain (a.a. 248-450) with a N-terminal hexa-Histidine tagDepositorInsertBeclin-1 ECD/BARA (BECN1 Human)
UseTagsHexa-HistadineExpressionBacterialMutationDNA optimized for E. coli expressionPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3
Plasmid#105802PurposeConcomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.DepositorTypeEmpty backboneUseFlp-in competentTagsORF1: 3C-mRuby3; ORF2: TEV-mClover3ExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 ORF3B_nostop
Plasmid#149320PurposeGateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertORF3B
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207 SARS-CoV2 TEV-NSP3
Plasmid#154402PurposeGateway-compatible Entry vectorDepositorInsertNSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of n…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceSept. 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV-2 NSP5 Cys146Ala_nostop
Plasmid#154030PurposeGateway-compatible Entry vectorDepositorInsertNSP5
UseGateway-compatible entry vector, with mutagenized…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJune 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Epac-S-H159
Plasmid#170347PurposemT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-PGK-Chst3
Plasmid#193024PurposeAAV vector plasmid expressing mouse chondroitin 6-sulfotransferase (Chst3) under the mouse phosphoglycerate kinase (PGK) promoterDepositorInsertChst3 (Chst3 Mouse)
UseAAVTags3xFLAG and myc-tagExpressionMammalianMutationPromoterPGKAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
FGFR1_HUMAN_D0
Plasmid#79719PurposeThis plasmid encodes the kinase domain of FGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertFGFR1 (FGFR1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 NSP1_nostop
Plasmid#149304PurposeGateway-compatible Entry vector, with insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertNSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207 SARS-CoV-2 E_nostop
Plasmid#149321PurposeGateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertE (E SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits