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Plasmid#31680DepositorInsertp21-activated protein kinase 2 (PAK2 Human)
UseLentiviralTagsM2ExpressionMammalianMutationPromoterAvailable sinceApril 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pILGFPH7
Plasmid#83554PurposeUsed to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.DepositorInsertADH2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterADH2Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro WT ATG3
Plasmid#26926DepositorInsertautophagy-related 3 (yeast) (Atg3 Mouse)
UseRetroviralTagsHis and V5ExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-hSt3Cas9 (pXK-1721)
Plasmid#208828PurposeCRISPR/hSt3Cas9 expression vector for Animal gene editing, harboring the humanized St3Cas9 gene and the corresponding optimized SP1.gRNA. Key Construct: mU6- SP1.gRNA-CMV-hSt3Cas9.DepositorTypeEmpty backboneUseCRISPR; Stcas9TagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-Cdc42 G15A
Plasmid#69356Purposeexpression of nucleotide free Cdc42 in bacterial cellsDepositorInsertCdc42 (CDC42 Human)
UseTagsGSTExpressionBacterialMutationG15APromotertacAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
LNCX BMP-RII
Plasmid#12641DepositorInsertBMP-RII (BMPR2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag-MBD1 R44Q-14-mVenusC
Plasmid#191394PurposeR44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenusDepositorInsertpcDNA3 Flag-MBD1 R44Q-14-mVenusC
UseTags3xFLAGExpressionMammalianMutationR44QPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-V5-PTPN14-del-N
Plasmid#61004PurposeExpresses human PTPN14 (del-FERM domain mutant) in mammalian cells, N-terminus V5 tagDepositorInsertPTPN14 delete N (PTPN14 Human)
UseTagsV5 tagExpressionMammalianMutationdeleted amino acids 17-310PromoterCMVAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NES-fRGECO1
Plasmid#125247PurposeExpresses red fluorescent calcium sensor in mammalian cellsDepositorInsertfRGECO2
UseTagsNES signalExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIVT/Smarca4
Plasmid#122146PurposeFor making Smarca4 RNADepositorInsertSmarca4 (Smarca4 Mouse)
UseFor in vitro transcriptionTagsExpressionMutationPromoterT7Available sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_SynP_DIO_tAKARα _WPRE
Plasmid#196675PurposeAAV plasmid for mammalian Cre-dependent expression of tAKARα (genetically encoded sensor for protein kinase A).DepositorInserttAKARα
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CRY2-CreN
Plasmid#75268PurposeThe second half of the photo-activatable cre recombinaseDepositorInsertsecond half of the cre recombinase fused to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-V5-PTPN14-del-C
Plasmid#61005PurposeExpresses human PTPN14 (del-phosphotase domain mutant) in mammalian cells, N-terminus V5 tagDepositorInsertPTPN14 delete C (PTPN14 Human)
UseTagsV5 tagExpressionMammalianMutationdeleted amino acids 934-1187PromoterCMVAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pegRNA_HEK4_20-7
Plasmid#201974PurposeExpress a pegRNA used for 20bp tag insertion at HEK4 site with a 7bp homology armDepositorInsertpegRNA_HEK4_20-7
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-H3C2
Plasmid#207780PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.DepositorArticleInsertsgRNA Targeting C-terminus of H3C2 (H3C2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K9R
Plasmid#84527PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationK(43,61,69,117,240,246,527,582,651)RPromoternoneAvailable sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
plko.1/shMCT4 #474
Plasmid#99597PurposeKnockdown of MCT4 expressionDepositorInsertMCT4 (SLC16A3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FLAG-BMAL1-S513A
Plasmid#186831PurposeExpresses FLAG-BMAL1-S513A in mammalian cellsDepositorInsertbmal1 (Bmal1 Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Serine 513 to AlaninePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FLAG-BMAL1-S515A
Plasmid#186832PurposeExpresses FLAG-BMAL1-S515A in mammalian cellsDepositorInsertbmal1 (Bmal1 Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Serine 515 to AlaninePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FLAG-BMAL1-S516A
Plasmid#186833PurposeExpresses FLAG-BMAL1-S516A in mammalian cellsDepositorInsertbmal1 (Bmal1 Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Serine 516 to AlaninePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only