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Addgene
Showing: 7481 - 7500 of 13941 results
  1. pBac[3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP]

    Plasmid
    #86454
    Purpose
    Nuclear reporter (histone-tagged EGFP) construct with the Tribolium specific ribosomal protein subunit 3 promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)
    Depositor
    Insert
    3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. mEos3.2-Nup50-N-10

    Plasmid
    #57479
    Purpose
    Localization: Nuclear Pore, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Insert
    Nup50 (NUP50 Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. mEos4b-Nup50-N-10

    Plasmid
    #57516
    Purpose
    Localization: Nuclear Pore, Excitation: 505 / 569, Emission: 516 / 581
    Insert
    Nup50 (NUP50 Human)
    Use
    Tags
    mEos4b
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. pX330-Flag-HeFSpCas9-H840A

    Plasmid
    #80456
    Purpose
    high fidelity enhanced SpCas9 mammalian expression vector
    Depositor
    Insert
    HeFSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
  5. pEGFP-C1-NuMA

    Plasmid
    #81029
    Purpose
    Expresses human NuMA with N-terminal EGFP tag in mammalian cells
    Depositor
    Insert
    Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  6. pCDH-CB-FLPe-P2A-tdTomato

    Plasmid
    #72259
    Purpose
    Express FLPe and tdTomato under CB promoter
    Depositor
    Insert
    FLPe-P2A-tdTomato
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    T230I in tdTomato (please see depositor's co…
    Promoter
    CB promoter and chicken beta-actin + CMV enhancer
    Availability
    Academic Institutions and Nonprofits only
  7. mTurquoise-Nesprin3-C-18

    Plasmid
    #55572
    Purpose
    Localization: Nuclear Membrane , Excitation: 434, Emission: 474
    Depositor
    Insert
    Nesprin3 (SYNE3 Human)
    Use
    Tags
    mTurquoise
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. 4YY8

    Plasmid
    #69961
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Insert
    PF13_0238:MAC052-B07:C233961
    Use
    Baculovirus expression
    Tags
    His and TEV cleavage site
    Expression
    Mutation
    Promoter
    polyhedrin
    Availability
    Academic Institutions and Nonprofits only
  9. 5C8G

    Plasmid
    #69968
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    Tb427tmp.01.1830:MAC054-B05:C234722
    Use
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Academic Institutions and Nonprofits only
  10. Cre Shine

    Plasmid
    #37404
    Purpose
    This is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.
    Depositor
    Insert
    Cre Shine
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  11. Top7 K39E K40E V48V K55E

    Plasmid
    #12464
    Purpose
    Plasmid contains Top7 K39E K40E V46A and K55E. Please note this is DIFFERENT than what the plasmid name implies.
    Depositor
    Insert
    Top7 K39E K40E V46A K55E
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    K39E K40E V46A K55E
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  12. pSBbi-Pur-AktAR2

    Plasmid
    #125195
    Purpose
    SB-transposon plasmid for stable expression of AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Insert
    AktAR2
    Use
    Transposon
    Tags
    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Availability
    Academic Institutions and Nonprofits only
  13. pSBbi-Puro_LPAR_AKAR-WT

    Plasmid
    #125206
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Insert
    LPAR_AKAR-WT
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Availability
    Academic Institutions and Nonprofits only
Showing: 7481 - 7500 of 13941 results