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Addgene
Showing: 7461 - 7480 of 13941 results

  1. mEmerald-Nesprin3-C-18

    Plasmid
    #54203
    Purpose
    Localization: Nuclear Membrane , Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Nesprin3 (SYNE3 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    54203-plasmid-map-sequence-id-247990

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  2. mCherry-NUP50-N-10

    Plasmid
    #55111
    Purpose
    Localization: Nuclear Pore, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    NUP50 (NUP50 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  3. p3E_he1a:ECFP

    Plasmid
    #113880
    Purpose
    p3E entry vector for Three Multisite Gateway
    Depositor
    Marnie Halpern
    Insert
    he1a (he1.1 Zebrafish)
    Use
    Zebrafish danio expression
    Tags
    ECFP
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    113880-plasmid-map-sequence-id-222569

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  4. pKP2185

    Plasmid
    #167635
    Purpose
    Express FepA D12C, I14P . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Insert
    fepA D12C, I14P
    Use
    Tags
    Expression
    Bacterial
    Mutation
    fepA with D12C, I14P mutations (Mutations are D34…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    167635-plasmid-map-sequence-id-339489

    Enlarge

  5. pKP2186

    Plasmid
    #167637
    Purpose
    Express FepA T13C, I14P . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Insert
    fepA T13C, I14P
    Use
    Tags
    Expression
    Bacterial
    Mutation
    fepA with T13C, I14P mutations (Mutations are T3…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    167637-plasmid-map-sequence-id-339561

    Enlarge

  6. pKP2187

    Plasmid
    #167640
    Purpose
    Express FepA V15C, I14P . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Insert
    fepA V15C, I14P
    Use
    Tags
    Expression
    Bacterial
    Mutation
    fepA with V15C, I14P mutations (Mutations are I36…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    167640-plasmid-map-sequence-id-339573

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  7. pKP2188

    Plasmid
    #167642
    Purpose
    Express FepA V16C, I14P . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Insert
    fepA V16C, I14P
    Use
    Tags
    Expression
    Bacterial
    Mutation
    FepA with V16C, I14P mutations. (They are numbere…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    167642-plasmid-map-sequence-id-337262

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  8. pKP2189

    Plasmid
    #167644
    Purpose
    Express FepA T17C, I14P . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Insert
    fepA T17C, I14P
    Use
    Tags
    Expression
    Bacterial
    Mutation
    fepA with T17C, I14P mutations (Mutations are I36…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    167644-plasmid-map-sequence-id-339582

    Enlarge

  9. pDSP23

    Plasmid
    #199372
    Purpose
    Encodes dual cistronic nuclear localized GCaMP6s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::GCaMP6s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    199372-plasmid-map-sequence-id-390928

    Enlarge

  10. pDSP31

    Plasmid
    #199373
    Purpose
    Encodes dual cistronic nuclear localized GCaMP6f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::GCaMP6f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    199373-plasmid-map-sequence-id-390590

    Enlarge

  11. pDSP32

    Plasmid
    #199374
    Purpose
    Encodes dual cistronic nuclear localized jGCaMP7s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::jGCaMP7s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    199374-plasmid-map-sequence-id-390801

    Enlarge

  12. pDSP33

    Plasmid
    #199375
    Purpose
    Encodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    199375-plasmid-map-sequence-id-391013

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  13. pCDH-CB-FLPe-P2A-tdTomato

    Plasmid
    #72259
    Purpose
    Express FLPe and tdTomato under CB promoter
    Depositor
    Kazuhiro Oka
    Insert
    FLPe-P2A-tdTomato
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    T230I in tdTomato (please see depositor's co…
    Promoter
    CB promoter and chicken beta-actin + CMV enhancer
    Availability
    Academic Institutions and Nonprofits only
    72259-plasmid-map-sequence-id-131837

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  14. 4YY8

    Plasmid
    #69961
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PF13_0238:MAC052-B07:C233961
    Use
    Baculovirus expression
    Tags
    His and TEV cleavage site
    Expression
    Mutation
    Promoter
    polyhedrin
    Availability
    Academic Institutions and Nonprofits only

  15. 5C8G

    Plasmid
    #69968
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Tb427tmp.01.1830:MAC054-B05:C234722
    Use
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Academic Institutions and Nonprofits only

  16. Top7 K39E K40E V48V K55E

    Plasmid
    #12464
    Purpose
    Plasmid contains Top7 K39E K40E V46A and K55E. Please note this is DIFFERENT than what the plasmid name implies.
    Depositor
    David Baker
    Insert
    Top7 K39E K40E V46A K55E
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    K39E K40E V46A K55E
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    12464-plasmid-map-sequence-id-235394

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  17. Cre Shine

    Plasmid
    #37404
    Purpose
    This is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.
    Depositor
    Thomas Hughes
    Insert
    Cre Shine
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    37404-plasmid-map-sequence-id-114840

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  18. pX330-Flag-HeFSpCas9-H840A

    Plasmid
    #80456
    Purpose
    high fidelity enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    HeFSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    80456-plasmid-map-sequence-id-151478

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  19. pEGFP-C1-NuMA

    Plasmid
    #81029
    Purpose
    Expresses human NuMA with N-terminal EGFP tag in mammalian cells
    Depositor
    Michael Mancini
    Insert
    Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    81029-plasmid-map-sequence-id-300392

    Enlarge

  20. WDR61

    Plasmid
    #28162
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WD repeat domain 61 (WDR61 Human)
    Use
    Baculovirus expression
    Tags
    His tag with TEV cleavage
    Expression
    Mutation
    Promoter
    polyhedrin
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 7461 - 7480 of 13941 results