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Showing: 1801 - 1850 of 40180 results

  2. pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai

    Plasmid
    #202568
    Purpose
    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    LINE-1
    Use
    Tags
    GFP-AI retrotransposition reporter and HaloTag7 o…
    Expression
    Mammalian
    Mutation
    Changed ORF1p Lysine 3 to Alanine, ORF1p Lysine 4…
    Promoter
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    202568-plasmid-map-sequence-id-429225

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  11. pSAG1::CAS9-U6::sg290860-6

    Plasmid
    #59855
    Purpose
    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.
    Depositor
    David Sibley
    Article
    Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229
    Insert
    CRISPR sg290860-6
    Use
    CRISPR; ; toxoplasma gondii
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    59855-plasmid-map-sequence-id-94714

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  25. pSK267

    Plasmid
    #129272
    Purpose
    "ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein
    Depositor
    Ahmad Khalil
    Article
    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi
    Insert
    NLS-VP16-ZF 43-8-NES
    Use
    Tags
    6xHA
    Expression
    Yeast
    Mutation
    Promoter
    SUP35
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    129272-plasmid-map-sequence-id-255863

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  34. pBEG R2-iCreT2ALuc-L3 #BGV208

    Plasmid
    #48992
    Purpose
    Supplies Cre recombinase and Luciferase and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).
    Depositor
    David Dankort
    Article
    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:
    Inserts
    cre recombinase
    Luciferase
    Use
    Luciferase; Gateway cloning vector
    Tags
    IRES (weak)
    Expression
    Mutation
    Promoter
    Unknown
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  40. Cenp-B DBD INCENP TSS/AAA mCherry

    Plasmid
    #45234
    Purpose
    Target INCENP (with TSS mutated to AAA) to centromeres via CENP-B DNA binding domain (DBD)
    Depositor
    Michael Lampson
    Article
    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.
    Insert
    Cenp-B DBD, INCENP-ΔCEN with AAA mutation, mCherry
    Use
    Tags
    CENP-B DBD, DNA binding domain of CENP-B and mChe…
    Expression
    Mammalian
    Mutation
    INCENP C-terminal TSS mutated to AAA
    Promoter
    t7
    Available since
    Sept. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    45234-plasmid-map-sequence-id-84485

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  42. pMM549

    Plasmid
    #112531
    Purpose
    pZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpR
    Depositor
    Timothy Lu
    Article
    Mimee et al Science. 2018 May 25;360(6391):915-918.
    Inserts
    HrtR
    ChuA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23107 and ProD
    Available since
    Feb. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    112531-plasmid-map-sequence-id-217579

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  47. pDGB3_alpha2_BeYDV geminino 2.0 (no ATG)-eGFP (GB5178)

    Plasmid
    #225443
    Purpose
    Geminino 2.0-no ATG on CDS. BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1.
    Depositor
    Diego Orzaez
    Insert
    BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225443-plasmid-map-sequence-id-447736

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  50. pSCL475

    Plasmid
    #219503
    Purpose
    Express Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in trans
    Depositor
    Seth Shipman
    Article
    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.
    Insert
    Ade2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL7
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219503-plasmid-map-sequence-id-435230

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Showing: 1801 - 1850 of 40180 results