MAP2-CD4
(Plasmid
#43916)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 43916 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFU-MAP2-Gateway
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Backbone manufacturerAddgene plasmid 43915
- Backbone size w/o insert (bp) 9600
- Total vector size (bp) 10900
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Vector typeMammalian Expression, Lentiviral
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsUse recA cells, such as STBL3 (Invitrogen)
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCD4
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SpeciesH. sapiens (human)
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Insert Size (bp)1300
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GenBank IDNM_000616.4 NP_000607.1
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Entrez GeneCD4 (a.k.a. CD4mut, IMD79, Leu-3, OKT4D, T4)
- Promoter Human MAP2 promoter
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byHuman CD4 cDNA sequence cloned from plasmid pMAC24-IRESII, Miltenyi Biotec,
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The MAP2-GFP lentiviral reporter plasmid pGZ-hMAP2 was purchased from System Biosciences (SR10047PA-1). The MAP2 promoter sequence from this plasmid was then cloned along with the Gateway cassette of pEF-DEST51 into the backbone of FUdeltaGW-rtTA (Addgene plasmid 19780) following removal of the ubiquitin promoter-rtTA sequence to produce FU-MAP2-Gateway (Addgene plasmid #43915).
This destination vector was subsequently used to generate MAP2-CD4 (human CD4 sequence cloned from pMACS4-IRESII, Miltenyi Biotec, Cambridge, MA).
The V5 and His tags present in the vector backbone are not expressed with the CD4 insert.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MAP2-CD4 was a gift from John Gearhart (Addgene plasmid # 43916 ; http://n2t.net/addgene:43916 ; RRID:Addgene_43916) -
For your References section:
Efficient conversion of astrocytes to functional midbrain dopaminergic neurons using a single polycistronic vector. Addis RC, Hsu FC, Wright RL, Dichter MA, Coulter DA, Gearhart JD. PLoS One. 2011;6(12):e28719. doi: 10.1371/journal.pone.0028719. Epub 2011 Dec 9. 10.1371/journal.pone.0028719 PubMed 22174877