pBEG R2-iCreT2ALuc-L3 #BGV208
(Plasmid
#48992)
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PurposeSupplies Cre recombinase and Luciferase and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48992 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBEG R2+L3
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Backbone manufacturerDankort Lab
- Total vector size (bp) 5692
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Vector typeLuciferase ; Gateway cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namecre recombinase
- Promoter Unknown
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Tag
/ Fusion Protein
- IRES (weak) (N terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer NA (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameLuciferase
- Promoter Unknown
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBEG R2-iCreT2ALuc-L3 #BGV208 was a gift from David Dankort (Addgene plasmid # 48992 ; http://n2t.net/addgene:48992 ; RRID:Addgene_48992) -
For your References section:
A modular lentiviral and retroviral construction system to rapidly generate vectors for gene expression and gene knockdown in vitro and in vivo. Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, Dankort D. PLoS One. 2013 Oct 11;8(10):e76279. doi: 10.1371/journal.pone.0076279. 10.1371/journal.pone.0076279 PubMed 24146852
Map uploaded by Addgene staff.