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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45176 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG(gateway)
- Total vector size (bp) 3400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepCAG-Brainbow3.0
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Speciesjelly fish and coral fluorescent proteins
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Insert Size (bp)3400
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer ACAAGTTTGTACAAAAAAGCAGGCT
- 3′ sequencing primer ACCACTTTGTACAAGAAAGCTGGGT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Addgene Notes
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A portion of this plasmid was derived from a plasmid made bycloned by Kim Cohen and Dawen Cai
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the backbone sequence is from a non Gateway pCAG vector and may be missing a few features. These sequencing mismatches do not affect plasmid function. Please refer to Addgene's restriction digest for accurate representation of the plasmid size.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-Brainbow3.0 was a gift from Joshua Sanes (Addgene plasmid # 45176 ; http://n2t.net/addgene:45176 ; RRID:Addgene_45176) -
For your References section:
Improved tools for the Brainbow toolbox. Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR. Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 2013 May 5. 10.1038/nmeth.2450 PubMed 23817127