Addgene

We narrowed to 3,174 results for: ado

Showing: 1441 - 1480 of 3174 results

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EF1a_NRL_P2A_Hygro_Barcode

Plasmid

#120469

Purpose

Barcoded lentiviral vector to express NRL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NRL (NRL Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEE062

Plasmid

#176819

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

701

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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mEmerald(luminal)-canxTM

Plasmid

#186961

Purpose

Expresses mEmerald connected with the N-terminus of Calnexin transmembrane domain.

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

CANX (CANX Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Only express calnexin (482-end) with ER signal pe…

Promoter

CMV Promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BE3-FNLS(RA)

Plasmid

#112671

Purpose

CMV expression vector for BE3-FNLS construct (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3-FNLS

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

NLS sequence at the N-terminus and D10A

Promoter

CMV

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRmine-eYFP-Kv2.1-WPRE

Plasmid

#130993

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-eYFP under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

EYFP-Kv2.1

Expression

Mutation

Promoter

hSyn

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc-NL-GW

Plasmid

#113446

Purpose

NanoLuc Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Luciferase; Gateway shuttle vector

Tags

cmyc-NanoLuc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pEE077

Plasmid

#176834

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

716

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-KIX (586-672)

Plasmid

#99336

Purpose

Bacterial expression of mouse CBP KIX domain

Depositor

Peter Wright

Article

Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

Insert

CBP KIX (586-672) (Crebbp Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 GW-NL-myc

Plasmid

#113447

Purpose

NanoLuc Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Luciferase; Gateway shuttle vector

Tags

NanoLuc-cmyc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pTwist-WIV1-CoV

Plasmid

#164438

Purpose

Encodes WIV1-CoV Spike Protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

WIV1-CoV Spike

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shSTIM1

Plasmid

#89816

Purpose

Knockdown human STIM1 expression

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

STIM1 shRNA (STIM1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_MESP1_P2A_Hygro_Barcode

Plasmid

#120459

Purpose

Barcoded lentiviral vector to express MESP1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MESP1 (MESP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCas9-beta-mmr

Plasmid

#169241

Purpose

Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Inserts

cas9
beta
mutL-E36K

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Changed glutamic acid 36 to lysine

Promoter

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti myc-NL-GW

Plasmid

#113454

Purpose

Lentiviral NanoLuc Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Lentiviral and Luciferase

Tags

cmyc-NanoLuc

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-FLAG-hEcto/Tif1g-CAmut

Plasmid

#20903

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Point mutagenesis of C125 and C128 to Alanine (RI…

Promoter

Available since

May 15, 2009

Availability

Academic Institutions and Nonprofits only
pLenti-TRE3G-BE3-PGK-Puro

Plasmid

#110845

Purpose

Lentiviral vector for dox-inducible expression of BE3 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Tags

Expression

Mutation

D10A

Promoter

TRE3G

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 GW-mCit-PA

Plasmid

#113449

Purpose

ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Gateway shuttle vector

Tags

mCitrine-ProteinA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a_ASCL5_P2A_Hygro_Barcode

Plasmid

#120430

Purpose

Barcoded lentiviral vector to express ASCL5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ASCL5 (ASCL5 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEE075

Plasmid

#176832

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

714

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-mCit-GW

Plasmid

#113448

Purpose

ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Gateway shuttle vector

Tags

ProteinA-mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-IgKL-mNG

Plasmid

#183465

Purpose

To produce AAV with the P3 promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

IgKL-mNeonGreen

Use

AAV

Tags

IgKL secretory signal

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRmine-eYFP-Kv2.1-WPRE

Plasmid

#130989

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-eYFP under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

EYFP-Kv2.1

Expression

Mutation

Promoter

CaMKIIa

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pFETCh_GABPA

Plasmid

#64253

Purpose

Homology Arms and 3X Flag with P2A Neo for 3' tagging of human GABPA

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

GABPA Homology Arms (GABPA Human)

Use

Tags

3X Flag P2A Neo

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-BE3RA-P2A-Puro

Plasmid

#110839

Purpose

Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA

Use

Lentiviral

Tags

Expression

Mutation

D10A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pAC147-pCR8-dCas9VP64

Plasmid

#48219

Purpose

dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP64 activation domain

Use

CRISPR; Gateway donor

Tags

HA Tag and VP64

Expression

Mutation

D10A;H840A

Promoter

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-gLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5

Plasmid

#125234

Purpose

Expresses SPARK oβ2AR transmembrane component in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

gLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5

Use

AAV

Tags

Flag, V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-BE3RA-PGK-Puro

Plasmid

#110843

Purpose

Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA

Use

Lentiviral

Tags

Expression

Mutation

D10A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCh_KMT2B

Plasmid

#101070

Purpose

Donor vector for 3' FLAG tag of human KMT2B

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KMT2B homology arms (KMT2B Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_KMT2B_2

Plasmid

#101075

Purpose

Encodes gRNA for 3' target of human KMT2B

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KMT2B (KMT2B Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_KMT2B_1

Plasmid

#101074

Purpose

Encodes gRNA for 3' target of human KMT2B

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KMT2B (KMT2B Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFIN-EF1a-GFP:2A:CHER(HA)-WPRE

Plasmid

#44597

Depositor

Susan Semple-Rowland

Article

Verrier et al PLoS One. 2011;6(5):e20553. doi: 10.1371

Insert

EF1a-GFP:2A:CHER(HA)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

human elongation factor -1a (pos 2528-3971)

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-Ctdnep1_Cter

Plasmid

#196522

Purpose

Entry vector for Gateway with Ctdnep1_Cter

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1-Cter (CTDNEP1 Human)

Use

Tags

Expression

Bacterial

Mutation

No transmembrane domain

Promoter

CMV promoter; T7 promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR201-Ctdnep1

Plasmid

#196520

Purpose

Entry vector for Gateway with Ctdnep1

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1-N1 (CTDNEP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

CMV promoter; T7 promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCH_ATF1

Plasmid

#64689

Purpose

Homology Arms and 3X Flag with P2A Neo for 3' tagging of human ATF1

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ATF1 Homology Arms (ATF1 Human)

Use

Tags

3X Flag P2A Neo

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
N-TAP A2A-YFP pcDNA3

Plasmid

#67852

Purpose

mammalian expression of A2A receptor with N terminal TAP tag and C terminal YFP fusion

Depositor

Christian Gruber

Article

Bergmayr et al J Biol Chem. 2013 Oct 4;288(40):28831-44

Insert

adenosine A2a receptor (ADORA2A Human)

Use

Tags

TAP and YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
pSP64T acvr1ba*

Plasmid

#185409

Purpose

Constitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

acvr1ba (acvr1ba Zebrafish)

Use

In vitro synthesis of mrna

Tags

Expression

Mammalian

Mutation

changed Threonine 206 to Aspartic acid

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti GW-mCit-PA

Plasmid

#113457

Purpose

Lentiviral ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Lentiviral

Tags

mCitrine-ProteinA

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIP-SFFV-GFP-RELA K5R

Plasmid

#196631

Purpose

Express GFP-RELA K5R

Depositor

Nicolas Manel

Article

Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

Insert

GFP-RELA K5R (RELA Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

K122R, K123R, K310R, K314R, K315R

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEE038

Plasmid

#176795

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

407

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEE048

Plasmid

#176805

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

504

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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