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Showing: 721 - 740 of 2137 results

  1. Rlucrarr3-Flash3

    Plasmid
    #74131
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 of Arrestin3.
    Depositor
    Louis Luttrell
    Article
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Insert
    Arrestin3
    Use
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Promoter
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  3. Rlucrarr3-Flash2

    Plasmid
    #74130
    Purpose
    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.
    Depositor
    Louis Luttrell
    Article
    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi
    Insert
    Arrestin3
    Use
    Tags
    Renilla luciferase (rLuc)
    Expression
    Mammalian
    Mutation
    Insertion of FlAsH motif (CCPGCC) following amino…
    Promoter
    Available since
    April 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. pBactin-AcGFP-Cdk5rap2-CTD

    Plasmid
    #196853
    Purpose
    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to AcGFP. Used to displace endogenous Cdk5rap2 from centrosomes.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    AcGFP-Cdk5rap2-CTD (Cdk5rap2 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196853-plasmid-map-sequence-id-390738

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  11. pAMPK alpha2 delta312X

    Plasmid
    #60127
    Purpose
    expresses constitutively active AMPKalpha2
    Depositor
    Morris Birnbaum
    Insert
    AMPK alpha 2 (Prkaa2 Rat)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Truncated at residue 312
    Promoter
    Available since
    Nov. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    60127-plasmid-map-sequence-id-317039

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  12. G protein alpha-s-mCherry

    Plasmid
    #66968
    Purpose
    G protein alpha-s internally tagged with mCherry and EE epitope
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1
    Insert
    G-alpha-s-EE-mCherry (Gnas Rat, Discosoma sp.)
    Use
    Tags
    internal EE epitope (residues 189-194 in alpha- s…
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    66968-plasmid-map-sequence-id-121428

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  16. pMT ERK1

    Plasmid
    #12656
    Depositor
    John Kyriakis
    Insert
    ERK1 (Mapk3 Rat)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 21, 2006
    Availability
    Academic Institutions and Nonprofits only

  20. pGP-AAV-syn-FLEX-jGCaMP7b-WPRE

    Plasmid
    #104493
    Purpose
    AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Has Service
    AAV Retrograde and AAV1
    Insert
    jGCaMP7b
    Use
    AAV and Cre/Lox
    Tags
    T7 epitope, Xpress tag, 6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    104493-plasmid-map-sequence-id-200290

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Showing: 721 - 740 of 2137 results