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pDR111_Bgal NLS

Plasmid

#188396

Purpose

Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Insert

lacZ

Use

Tags

PhoD secretion peptide and SV40 NLS

Expression

Bacterial

Mutation

Promoter

Phyper spank

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pOCC61

Plasmid

#118877

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3C

Depositor

PEPC MPI CBG, Marc Bickle

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette

Use

Tags

mGFP, HIS6, cleavable with 3C protease

Expression

Insect

Mutation

Promoter

polH

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pRDA_085

Plasmid

#158583

Purpose

Lentiviral expression of SpCas9; P2A site provides mKate2

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Insert

SpCas9

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBK-EQR-BE3

Plasmid

#85172

Purpose

Expresses EQR-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

EQR-BE3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Bin1b

Plasmid

#109509

Purpose

Muscle specific zebrafish expression of mKate2 fused to Bin1b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Bin1b (bin1b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pShuttle

Plasmid

#192871

Purpose

Gateway compatible vector with BsaI cloning site

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Type

Empty backbone

Use

Gateway-compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCDH_TNF-SBP-EGFP

Plasmid

#65282

Purpose

TNF reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Use

Lentiviral

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mutation

Promoter

CMV

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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Brachyury-eGFP

Plasmid

#21063

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

Brachyury promoter

Use

Lentiviral

Tags

eGFP

Expression

Mutation

Promoter

Available since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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KHBD00222

Plasmid

#34370

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1832 (CG1832 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pPbU6_hdhfr/yfcu_Cas9

Plasmid

#216423

Purpose

Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.

Depositor

Ellen Bushell

Article

Jonsdottir et al bioRxiv 2024.04.20.590404

Type

Empty backbone

Use

CRISPR; Expression in plasmodium berghei

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-2xFYVE_hrs

Plasmid

#67676

Purpose

Multisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrs

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

2xFYVEhrs

Use

Gateway multisite 3' entry clone

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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EF1a_SPIB_P2A_Hygro_Barcode

Plasmid

#120483

Purpose

Barcoded lentiviral vector to express SPIB in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SPIB (SPIB Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 PAK5

Plasmid

#60532

Purpose

Donor vector for human Pak5

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pGWB410

Plasmid

#74804

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB545

Plasmid

#74879

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-TM-CIBN-BLITz1-TetR-VP16

Plasmid

#89878

Purpose

expresses TM-CIBN-BLITz1-TetR-VP16 of BLITz system

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

TM-CIBN-TevN-BLITz1-TetR-VP16

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Aalb_743

Plasmid

#176662

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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IY601: pMVP/Lenti/Blast-DEST

Plasmid

#121849

Purpose

pMVP destination vector, empty Lentivirus vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Lentiviral; Pmvp gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLL3.7-K122-FH-TAZ-TEAD-p-H2B-mCherry

Plasmid

#139649

Purpose

Reporter assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

H2B-mCherry

Use

Lentiviral

Tags

FLAG, His6

Expression

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ST-SBP-tagBFP

Plasmid

#65266

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)

Use

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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