pDONR223 PAK5
(Plasmid
#60532)
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PurposeDonor vector for human Pak5
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 60532 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR223
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5005
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Vector typeGateway Donor vector
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePak5
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Alt namePak7
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SpeciesH. sapiens (human)
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Insert Size (bp)2160
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Entrez GenePAK5 (a.k.a. PAK7)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is derived from Addgene plasmid #23850. We changed a1208c, g79c to align with pubmed sequence and added a stop codon.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR223 PAK5 was a gift from John Brognard (Addgene plasmid # 60532 ; http://n2t.net/addgene:60532 ; RRID:Addgene_60532) -
For your References section:
Targeted genetic dependency screen facilitates identification of actionable mutations in FGFR4, MAP3K9, and PAK5 in lung cancer. Fawdar S, Trotter EW, Li Y, Stephenson NL, Hanke F, Marusiak AA, Edwards ZC, Ientile S, Waszkowycz B, Miller CJ, Brognard J. Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12426-31. doi: 10.1073/pnas.1305207110. Epub 2013 Jul 8. 10.1073/pnas.1305207110 PubMed 23836671