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Addgene
Showing: 7341 - 7360 of 13941 results

  1. C24m

    Plasmid
    #120964
    Purpose
    MoClo golden gate assembly CD part for lambda cI (repressor of pL or pOR1-OR2; use C64m for temperature-sensitive version). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    lambda-cI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120964-plasmid-map-sequence-id-236100

    Enlarge

  2. C31m

    Plasmid
    #120966
    Purpose
    MoClo golden gate assembly CD part for Bxb1 integrase. See DOI: 10.1007/s00438-006-0129-5. Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    bxb1 integrase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120966-plasmid-map-sequence-id-236888

    Enlarge

  3. C40m

    Plasmid
    #120973
    Purpose
    MoClo golden gate assembly CD part for blank part (random 50bp sequence) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Blank (Random 50bp sequence)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120973-plasmid-map-sequence-id-236027

    Enlarge

  4. C59m

    Plasmid
    #120976
    Purpose
    MoClo golden gate assembly CD part for mScarlet (RFP variant; see https://www.fpbase.org/protein/mscarlet/). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mScarlet
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120976-plasmid-map-sequence-id-236030

    Enlarge

  5. C63m

    Plasmid
    #120979
    Purpose
    MoClo golden gate assembly CD part for LacI (Lac repressor; identical to C1d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    lacI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120979-plasmid-map-sequence-id-236033

    Enlarge

  6. C80m

    Plasmid
    #120992
    Purpose
    MoClo golden gate assembly CD part for mfLon protease (see doi: [10.1038/nbt.3053]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mfLon protease
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120992-plasmid-map-sequence-id-236095

    Enlarge

  7. C84m

    Plasmid
    #120995
    Purpose
    MoClo golden gate assembly CD part for sigma28/RpoF (RNA polymerase required for flagellar formation in E. coli). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Sigma 28
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120995-plasmid-map-sequence-id-236105

    Enlarge

  8. C115m

    Plasmid
    #121014
    Purpose
    MoClo golden gate assembly CD part for TP901 integrase (Integrase from lactococcal phage TP901). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    TP901 integrase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121014-plasmid-map-sequence-id-236321

    Enlarge

  9. HA-CPI-17-T38E-NLS

    Plasmid
    #100778
    Purpose
    Mammalian expression of CPI-17 phospho-mimetic nuclear
    Depositor
    Joseph Gordon
    Insert
    CPI-17-T38E-NLS (PPP1R14A Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    T38E
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    100778-plasmid-map-sequence-id-193198

    Enlarge

  10. HA-CPI-17-T38A-NLS

    Plasmid
    #100780
    Purpose
    Mammalian expression of CPI-17 neutral mutant nuclear
    Depositor
    Joseph Gordon
    Insert
    CPI-17-T38A-NLS (PPP1R14A Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    T38A
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    100780-plasmid-map-sequence-id-193203

    Enlarge

  11. pOpen-NotIR (GC)

    Plasmid
    #165507
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    NotI (GC-rich recognition site)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    165507-plasmid-map-sequence-id-338586

    Enlarge

  12. pOpen-EcoRIM

    Plasmid
    #165515
    Purpose
    Methylase recognizes double-stranded sequence GAATTC, methylates A-3 on both strands, and protects DNA from cleavage by EcoRI.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    M.EcoRI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    165515-plasmid-map-sequence-id-338645

    Enlarge

  13. pOpen-PstIM

    Plasmid
    #165536
    Purpose
    Methylase that recognizes the dsDNA sequence CTGCAG, methylates on A-5 on both strands, and protects DNA from cleavage by PstI.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    M.PstI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    165536-plasmid-map-sequence-id-339126

    Enlarge

  14. pFBOH-LIC

    Plasmid
    #26099
    Purpose
    SGC Empty baculovirus expression/transfer vector
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    6x His and Thrombin protease cleavage site
    Expression
    Mutation
    Promoter
    polyhedrin
    Availability
    Industry, Academic Institutions, and Nonprofits
    26099-plasmid-map-sequence-id-12255

    Enlarge

  15. pETcon(I-LtrI)

    Plasmid
    #41523
    Depositor
    Andrew Scharenberg
    Insert
    I-LtrI
    Use
    Tags
    3x(G4S) Linker, G3S Linker, HA Tag, and Myc Tag
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Availability
    Academic Institutions and Nonprofits only
    41523-plasmid-map-sequence-id-59600

    Enlarge

  16. Nesprin3-C-18

    Plasmid
    #58194
    Purpose
    Localization: Nuclear Membrane , Excitation: N/A, Emission: N/A
    Depositor
    Michael Davidson
    Insert
    Nesprin3 (SYNE3 Human)
    Use
    Tags
    SNAP tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. Dendra2-LaminA-C-18

    Plasmid
    #57727
    Purpose
    Localization: Nuclear Envelope, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Insert
    LaminA
    Use
    Tags
    Dendra2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. mEmerald-Nup35-C-18

    Plasmid
    #54207
    Purpose
    Localization: Nuclear Pore, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Nup35 (NUP35 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. mEmerald-Nup35-N-14

    Plasmid
    #54208
    Purpose
    Localization: Nuclear Pore, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Nup35 (NUP35 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. mEmerald-Nup50-C-10

    Plasmid
    #54209
    Purpose
    Localization: Nuclear Pore, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Nup50 (NUP50 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 7341 - 7360 of 13941 results