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Plasmid#38132DepositorInsertMITF-A (MITF Human)
UseTagsEGFPExpressionMammalianMutationdeleted stop codonPromoterCMVAvailable sinceJuly 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-MITF-M
Plasmid#38131DepositorInsertMITF-M (MITF Human)
UseTagsEGFPExpressionMammalianMutationdeleted stop codonPromoterCMVAvailable sinceJuly 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLVX_DSB_Spectrum-V3
Plasmid#192476PurposeLentiviral DSB Spectrum V3 Reporter SystemDepositorInsertBFP1.2, mCherry, incompleteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
BPK764
Plasmid#65767PurposeBacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNADepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
UseCRISPRTags3x FLAG and NLSExpressionBacterialMutationPromoterT7 (x2)Available sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCGN-HCF-1 fl
Plasmid#53309Purposeplasmid contains human HCFC1 full length gene with HA and c-myc tagsDepositorInsertHCFC1 (HCFC1 Human)
UseTagsHA and c-mycExpressionMammalianMutationPromoterCMVAvailable sinceMay 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pR26 CAG AsiSI/MluI
Plasmid#74286PurposeGene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or MluiDepositorInsertRosa26 5-homology region
UseTagsExpressionMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMVHA E2F1
Plasmid#24225PurposeMammalian expression of human E2F1 with HA tagDepositorInsertE2F1 (E2F1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-GCaMP6s
Plasmid#111262PurposeFor enriched expression of GCaMP6s in axonsDepositorHas ServiceAAV1Insertaxon-GCaMP6s
UseAAVTagsGAP43 palmitoylation domainExpressionMammalianMutationPromoterhSynapsin1Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-FLAG-ATBF1
Plasmid#40927DepositorInsertATBF1 (ZFHX3 Human)
UseTagsFLAGExpressionMammalianMutationSee depositor comments below.PromoterCMVAvailable sinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.Cre
Plasmid#170367PurposeAAV vector expressing Cre under human synapsin promotorDepositorInsertCre
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spRYCas9-ABE-UMax
Plasmid#222139PurposeNear PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafishDepositorInsertTadA-nSpRYCas9
UseCRISPR; Zebrafish expressionTagsbipartite NLSExpressionMutationPromoterT3Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPUR-mU6-sgRNA-2XPP7
Plasmid#194504PurposeEmpty backbone for sgRNA-2XPP7 in CRIPSR FISHer systemDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRY2PHR-mCherryN1
Plasmid#26866PurposeExpresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modulesDepositorInsertCRY2PHR (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4_17G6-1 heavy chain
Plasmid#198249Purposerecombinant expression of heavy chain of monoclonal anti-AMP antibody 17G6-1DepositorInsertheavy chain of monoclonal anti-AMP antibody 17G6-1 (mouse IgG2b)
UseTagsIL10 signal peptideExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.flex.H2B.GFP
Plasmid#170368PurposeCre-inducible AAV vector expressing nuclear GFP.DepositorInsertflex H2B GFP
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
PLIN2-V5-APEX2
Plasmid#170573PurposeA C-terminal APEX2 fusion to the mouse perilipin-2 protein PLIN2DepositorInsertPLIN2 (Plin2 Mouse)
UseLentiviralTagsV5-APEX2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS U6a gRNA
Plasmid#158961PurposegRNA backbone driven by zebrafish U6a promoterDepositorInsertszf U6a promoter
gRNA backbone
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt
Plasmid#194519PurposeDual fluorescence reporter for studying protein degradationDepositorInsertTIF4631 leader sequence
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA Ef1alpha Puro-T2A-GFP
Plasmid#111596PurposesgRNA Ef1alpha Puro-T2A-GFPDepositorInsertmU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only