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Addgene
Showing: 7321 - 7340 of 13941 results

  1. tdTomato-FTR-940

    Plasmid
    #58094
    Purpose
    Localization: Nuclear - Nucleolus, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    FTR
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  2. tdTomato-FTR-952

    Plasmid
    #58095
    Purpose
    Localization: Nuclear - Nucleolus, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    FTR
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  3. pOpen-9N7polA

    Plasmid
    #165500
    Purpose
    Thermophilic DNA polymerase; genetically engineered to have a decreased 3'-5' proofreading exonuclease activity
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    9 degrees N-7 DNA polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    165500-plasmid-map-sequence-id-338428

    Enlarge

  4. pOpen-Eco31I

    Plasmid
    #165506
    Purpose
    Type II restriction enzyme that recognized the double-stranded sequence. GGTCTC (N) and cleaves after N-1; isoschizomer of BsaI.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    Eco31I
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    165506-plasmid-map-sequence-id-338512

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  5. P9m

    Plasmid
    #120937
    Purpose
    MoClo golden gate assembly AB part for pOR1-OR2 (lambda cI-repressable strong promoter) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Pr-OR1-OR2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120937-plasmid-map-sequence-id-247706

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  6. P18m

    Plasmid
    #120941
    Purpose
    MoClo golden gate assembly AB part for T7 promoter (strong, T7 polymerase-dependent constitutive promoter; use with C95m). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    T7
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120941-plasmid-map-sequence-id-235947

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  7. C32m

    Plasmid
    #120967
    Purpose
    MoClo golden gate assembly CD part for CinR (Quorum-sensing receptor for 3OC14). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohCD carb CinR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120967-plasmid-map-sequence-id-236380

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  8. C36m

    Plasmid
    #120970
    Purpose
    MoClo golden gate assembly CD part for RhlI (quorum-sensing synthase for 3OC14). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohCD carb RhlI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120970-plasmid-map-sequence-id-245423

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  9. C86m

    Plasmid
    #120997
    Purpose
    MoClo golden gate assembly CD part (phage lysis protein; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    X174E
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120997-plasmid-map-sequence-id-236126

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  10. pSV40_HA-mPACT

    Plasmid
    #120537
    Purpose
    protein expression
    Depositor
    Petr Svoboda
    Insert
    PACT (Prkra Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120537-plasmid-map-sequence-id-233981

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  11. pOpen-UDG

    Plasmid
    #165531
    Purpose
    Uracil DNA glycosylase catalyzes the hydrolysis of the N-glycosidic bond from deoxyuridine to release uracil.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    UDG
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    165531-plasmid-map-sequence-id-339117

    Enlarge

  12. pOpen-T4 PGD

    Plasmid
    #165550
    Purpose
    T4 pyrimidine DNA glycosylase; bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    T4 PGD (T4 Endonuclease V)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    165550-plasmid-map-sequence-id-339867

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  13. pEGGsH6-MCS_SHORT_inFrame

    Plasmid
    #211438
    Purpose
    Vector backbone for random polymerase chain reaction screen
    Depositor
    Helmut Pospiech
    Insert
    MCS-tev cleavage site-EGFP-6xHis-Tag
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211438-plasmid-map-sequence-id-420494

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  14. HA-CPI-17-T38E-NLS

    Plasmid
    #100778
    Purpose
    Mammalian expression of CPI-17 phospho-mimetic nuclear
    Depositor
    Joseph Gordon
    Insert
    CPI-17-T38E-NLS (PPP1R14A Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    T38E
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    100778-plasmid-map-sequence-id-193198

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  15. HA-CPI-17-T38A-NLS

    Plasmid
    #100780
    Purpose
    Mammalian expression of CPI-17 neutral mutant nuclear
    Depositor
    Joseph Gordon
    Insert
    CPI-17-T38A-NLS (PPP1R14A Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    T38A
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    100780-plasmid-map-sequence-id-193203

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  16. p15TV-L

    Plasmid
    #26093
    Purpose
    SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Industry, Academic Institutions, and Nonprofits
    26093-plasmid-map-sequence-id-52817

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  17. pMLLAC-J72041

    Plasmid
    #26186
    Depositor
    Christopher Anderson
    Insert
    Tev Protease Cleavage site
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    26186-plasmid-map-sequence-id-12307

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  18. NET29B.mm pRRLSIN (1744)

    Plasmid
    #62053
    Purpose
    lentiviral expression of nuclear envelope transmembrane protein
    Depositor
    Eric Schirmer
    Insert
    NET29B (Tmem120b Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Availability
    Academic Institutions and Nonprofits only
    62053-plasmid-map-sequence-id-101297

    Enlarge

  19. pOpen-NotIR (GC)

    Plasmid
    #165507
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    NotI (GC-rich recognition site)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    165507-plasmid-map-sequence-id-338586

    Enlarge

  20. P30m

    Plasmid
    #120942
    Purpose
    MoClo golden gate assembly AB part for pL-lacO1 (LacI-repressed promoter, as used in DOI:10.1038/35002125). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    pL-lacO1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120942-plasmid-map-sequence-id-238038

    Enlarge

Showing: 7321 - 7340 of 13941 results