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pU6-SAG1-DHFR

Plasmid

#80322

Purpose

Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

SAG1 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pDA025

Plasmid

#131384

Purpose

ORFeus expression, mutant

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Use

Tags

ORF2-3xFlag

Expression

Mammalian

Mutation

H230A En

Promoter

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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Accessory plasmid 1

Plasmid

#128569

Purpose

for mixing with SPECS library to produce lentivirus

Depositor

Timothy Lu

Article

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Insert

ECFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#112922

Purpose

Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB413

Plasmid

#74807

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pATLIC

Plasmid

#110287

Purpose

A backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)

Depositor

In-Geol Choi

Article

Ko et al Appl Environ Microbiol. 2012 May;78(9):3

Insert

yfaL

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB412

Plasmid

#74806

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1neo-NLucYAP15

Plasmid

#107610

Purpose

measure LATS activity by Luciferase Assay

Depositor

Xiaolong Yang

Article

Azad et al Nat Commun. 2018 Mar 13;9(1):1061. doi:

Insert

NLucYAP15

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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LB901: pMVP/PB/Blast-DEST

Plasmid

#121873

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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NylB'-SCY_pET21b

Plasmid

#215417

Purpose

Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylB'-SCY

Use

Tags

Expression

Bacterial

Mutation

R187S/F264C/D370Y

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pVEX-CC1

Plasmid

#74170

Purpose

protein expression of chicken DCTN1 p150Glued AA 217-548 (CC1) in bacteria

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

DCTN1 (DCTN1 Chicken)

Use

Tags

None

Expression

Bacterial

Mutation

Promoter

Inducible Tac promoter

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only
pGWB408

Plasmid

#74802

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB415

Plasmid

#74809

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pAT9650-BEAR-GFP-preedited

Plasmid

#162992

Purpose

BEAR control plasmid with split EGFP and intact 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LB701: pMVP/PB/mCherry-DEST

Plasmid

#121871

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ mCherry selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBK-YE1-BE3

Plasmid

#85174

Purpose

Expresses YE1-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

YE1-BE3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-WWC2

Plasmid

#172995

Purpose

constitutive expression of FLAG-tagged WWC2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-WWC2 (WWC2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pBK-YEE-BE3

Plasmid

#85177

Purpose

Expresses YEE-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

YEE-BE3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1neo-14-3-3-CLuc

Plasmid

#107611

Purpose

measure LATS activity by Luciferase Assay

Depositor

Xiaolong Yang

Article

Azad et al Nat Commun. 2018 Mar 13;9(1):1061. doi:

Insert

pCDNA3.1hygro-BamHI-CLuc1433-NotI

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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pFRTpsiCHECK_Gaussia_luciferase

Plasmid

#154459

Purpose

Expression of gaussia luciferase and firefly luciferase

Depositor

Markus Landthaler

Article

Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

Insert

Gaussia luciferase

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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