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Addgene
Showing: 7281 - 7300 of 13941 results

  1. pOP5TT

    Plasmid
    #112613
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and thioredoxin tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-thioredoxin and Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112613-plasmid-map-sequence-id-220251

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  2. pOpen-ECOpolA

    Plasmid
    #165532
    Purpose
    DNA polymerase that also exhibits 3'-5' and 5'-3' exonuclease activity.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    E. coli DNA Polymerase I
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    165532-plasmid-map-sequence-id-339121

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  3. pOpen-Pwopol

    Plasmid
    #165562
    Purpose
    DNA polymerase; additionally exhibits 3' to 5' exonuclease activity.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    Pwo DNA Polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    165562-plasmid-map-sequence-id-340035

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  4. pET15b (I-WcaINhis) K125I

    Plasmid
    #195619
    Purpose
    Expresses I-WcaI homing endonuclease from Wickerhamomyces canadensis containing the K125I mutation
    Depositor
    Frederick Gimble
    Insert
    I-WcaI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A2030T, A2031T
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    195619-plasmid-map-sequence-id-384567

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  5. pET15b (I-WcaINhis) K226I

    Plasmid
    #195620
    Purpose
    Expresses I-WcaI homing endonuclease from Wickerhamomyces canadensis containing the K226I mutation
    Depositor
    Frederick Gimble
    Insert
    I-WcaI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A1930T, A1931T
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    195620-plasmid-map-sequence-id-384568

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  6. pHS2

    Plasmid
    #81037
    Purpose
    HO endonuclease expression
    Depositor
    John McCusker
    Insert
    HYGMX
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF
    Availability
    Academic Institutions and Nonprofits only
    81037-plasmid-map-sequence-id-274379

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  7. Zahra73-59

    Plasmid
    #23957
    Depositor
    Lawrence Cohen
    Insert
    VSP (tpte Zebrafish)
    Use
    Tags
    Cerulean and Citrulline
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    23957-plasmid-map-sequence-id-11261

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  8. Zahra

    Plasmid
    #23958
    Depositor
    Lawrence Cohen
    Insert
    VSP (tpte Zebrafish)
    Use
    Tags
    Cerulean and Citrulline
    Expression
    Mammalian
    Mutation
    R153Q
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    23958-plasmid-map-sequence-id-11262

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  9. Zahra76-77

    Plasmid
    #23959
    Depositor
    Lawrence Cohen
    Insert
    VSP (tpte Zebrafish)
    Use
    Tags
    Cerulean and Citrulline
    Expression
    Mammalian
    Mutation
    T156R
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    23959-plasmid-map-sequence-id-11263

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  10. pET28a-LIC

    Plasmid
    #26094
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Hexahistidine tag with Thrombin cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    26094-plasmid-map-sequence-id-246717

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  11. pET28-MHL

    Plasmid
    #26096
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    Hexahistidine tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Industry, Academic Institutions, and Nonprofits
    26096-plasmid-map-sequence-id-326568

    Enlarge

  12. pOpen-EcoRIR

    Plasmid
    #165504
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    EcoRI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    165504-plasmid-map-sequence-id-338511

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  13. pOpen-HindIIIR

    Plasmid
    #165508
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence AAGCTT and cleaves after A-1.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    HindIII
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    165508-plasmid-map-sequence-id-338587

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  14. C9m

    Plasmid
    #120958
    Purpose
    MoClo golden gate assembly CD part for deCFP (cyan fluorescent protein) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    deCFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120958-plasmid-map-sequence-id-235973

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  15. C76m

    Plasmid
    #120988
    Purpose
    MoClo golden gate assembly CD part for KanR (kanamycin resistance protein). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    KanR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120988-plasmid-map-sequence-id-236053

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  16. C77m

    Plasmid
    #120989
    Purpose
    MoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin resistance protein). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    AmpR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120989-plasmid-map-sequence-id-236056

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  17. C78m

    Plasmid
    #120990
    Purpose
    MoClo golden gate assembly CD cloning part for ChlorR (chloramphenicol resistance protein). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ChlorR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120990-plasmid-map-sequence-id-236059

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  18. U4m

    Plasmid
    #121017
    Purpose
    MoClo golden gate assembly BC part for UTR1 (very strong RBS). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    UTR1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121017-plasmid-map-sequence-id-236179

    Enlarge

Showing: 7281 - 7300 of 13941 results