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Plasmid#138495PurposeA-to-G base editorDepositorInsertecTadA(8e V106W)-nSpCas9
UseTagsExpressionMammalianMutationTadA-8e V106WPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase
Plasmid#119816Purpose3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cellsDepositorInsertsLuciferase
Puromycin
UseLentiviral and LuciferaseTagsEGFP and V5ExpressionMammalianMutationStop codon removed in the frame of the V5 frame v…PromoterCMV/TO and Human Phosphoglycerate kinaseAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Hs.cargo(Cas9)
Plasmid#174864PurposeExpresses human Peg10 cargoRNA encoding SpCas9DepositorInsertSpCas9 flanked by HsPeg10 UTRs
UseTagsHA-SV40 NLS and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF3370
Plasmid#144846PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMYBL1 (MYBL1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
AiP1924 - pAAV-AiE2543m-minBG-SYFP2-WPRE-BGHpA
Plasmid#214583PurposeAiE2543m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsInsertSYFP2
UseAAVTagsExpressionMutationPromoterminBGAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKIKOarsBKm
Plasmid#46766PurposeE.coli genomic integration at arsBDepositorTypeEmpty backboneUseE.coli genomic integrationTagsExpressionMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
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EGFP-Tau(WT)
Plasmid#187023PurposeExpresses EGFP tagged human 2N4R tau (WT) in mammalian cells with CMV promoterDepositorInsertMAPT (MAPT Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZR071_SFFV-dCas9-mCherry-KRAB
Plasmid#180264PurposeLentiviral expression vector for dCas9-KRAB in mammalian cellsDepositorInsertdCas9
UseCRISPR and LentiviralTagsKRAB and mCherryExpressionMammalianMutationNuclease dead Cas9PromoterSFFVAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-XBP1 mNeonGreen NLS
Plasmid#115968PurposeER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreenDepositorInsertX-box binding protein 1 (XBP1 Synthetic, Human)
UseLentiviralTagsHA tag, c-myc NLS, and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF
Plasmid#131710PurposeMammalian Expression of Flag tagged BRAFDepositorInsertBRAF (BRAF Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJR85
Plasmid#140095PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.DepositorInsertsgRNA with cs1 in stem loop 2 of the constant region
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G
Plasmid#167935PurposeInducible CRISPRi expressionDepositorInsertKRAB-dCas9-DHFR
UseLentiviralTagsExpressionMutationPromotertetONAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAY5 (mAID-EGFP-NLS piggyBac)
Plasmid#140532PurposemAID-EGFP-NLS piggyBacDepositorInsertmAID-EGFP-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAG-BFP-sesRNA-GFP-WPRE-pA
Plasmid#192063PurposeExpression of BFP, sesRNA in mammalian cells, with GFP as efRNA.DepositorInsertsesRNA for tdTom
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9-VPR_P2A_mCherry
Plasmid#154193PurposeLentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry markerDepositorInsertsp-dCas9-VPR_P2A_mCherry
UseLentiviralTagsExpressionMammalianMutationD10A, H840APromoterEF1asAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Bxb1
Plasmid#182142PurposeExpression of human codon optimized Bxb1DepositorInserthuman codon optimized Bxb1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadDE
Plasmid#193837PurposeExpress TadDE (with SpCas9) in mammalian cellsDepositorInsertTadA-DualEditor-Cas9D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_VP35_EBOV
Plasmid#103050PurposeEncodes Ebola virus VP35 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorInsertVP35 gene of Ebola virus (VP35 (Zaire ebolavirus, Mayinga))
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_L_EBOV
Plasmid#103052PurposeEncodes Ebola virus L gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorInsertL gene of Ebola virus (L Zaire ebolavirus, Mayinga)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_VP30_EBOV
Plasmid#103051PurposeEncodes Ebola virus VP30 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorInsertVP30 gene of Ebola virus (VP30 Zaire ebolavirus, Mayinga)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pimEJ5GFP
Plasmid#44026DepositorInsertEJ5GFP egfp-based chromosomal break reporter
UseTagsExpressionMammalianMutationpCAGGS promoter and eGFP separated by pgkPURO cas…PromoterpCAGGSAvailable sinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 GFP-h-PKD2
Plasmid#83451PurposeGFP-tagged PC2 for Eukaryotic expression (883)DepositorInsertPolycystin-2 (PKD2 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/mStayGold(c4)=UtrCH
Plasmid#212020PurposeFor filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).DepositorInsertUtrCH
UseTagsmStayGold(c4)ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8001 - pCMV-R8.91 (D64V)
Plasmid#202687PurposeR8.91 plasmid with mutated integrase (D64V)DepositorInsertgag pol
UseLentiviralTagsExpressionMammalianMutationD64VPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET His6 TEV LIC cloning vector (1B)
Plasmid#29653DepositorTypeEmpty backboneUseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hUL
Plasmid#27080PurposeIntegration-free (episomal) expression of human L-MYC and LIN28DepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
lentiMPH v2
Plasmid#89308Purposelenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.DepositorInsertMS2-P65-HSF1_2A_Hygro (RELA Synthetic, Human)
UseLentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1aAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-WT
Plasmid#17608PurposeMammalian expression of HA tagged ubiquitinDepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
TurboID-His6_pET21a
Plasmid#107177Purposeexpresses His6-tagged TurboID in bacteriaDepositorInsertTurboID (BirA mutant)
UseTagsExpressionBacterialMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GenEPi
Plasmid#140236PurposeMammalian expression of Piezo1-based fluorescent force sensor GenEPiDepositorInsertGenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)
UseTagsGCaMP 6s RS1 EF4ExpressionMammalianMutationPromotersimian CMV IE94Available sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)
Plasmid#101139PurposeLuciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mCherry
Plasmid#41583DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam
Plasmid#160451Purposevector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pink Flamindo
Plasmid#102356Purposebiosensor for cAMP in mammalian cellsDepositorInsertPink Flamindo
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.S
Plasmid#103006Purposenon-standard AAV2 rep-AAV-PHP.S cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.S VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
gag-P3-pol
Plasmid#211374PurposePackaging plasmid for v3b PE-eVLP productionDepositorInsertMMLVgag-P3-pol
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2
Plasmid#41843DepositorInsertCD20
UseTagsHA and c-mycExpressionYeastMutationPromoterAvailable sinceJan. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
rAAV2-retro helper
Plasmid#81070Purposeretrograde AAV helper vector, carrying rep and modified capsid geneDepositorInsertsrep
rAAV2-retro cap
UseAAV and Unspecified; Helper vectorTagsExpressionMutationPro2Ala, possibly Asp613Gly and changes made to A…PromoterAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
BirA pLEMO
Plasmid#119818PurposeExpress endogenous biotin ligase in E.coliDepositorInsertBiotin ligase
UseTagsExpressionBacterialMutationPromoterRhamnose promoterAvailable sinceApril 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
P4-PE
Plasmid#211375PurposePackaging plasmid for v3b PE-eVLP productionDepositorInsertP4-PE
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKeima-LiveDrop
Plasmid#199695Purposelipophagy reporter system based on the Keima-fluorophoreDepositorInsertGPAT4 (GPAT4 Human)
UseTagsKeimaExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDx_mScarlet3
Plasmid#189754PurposeDual expression vector for bacteria and mammalian cells producing mScarlet3 red fluorescent proteinDepositorInsertmScarlet3
UseTags6xHisExpressionBacterial and MammalianMutationPromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_NSP4ss_SerM_NucA-His
Plasmid#218190PurposeBacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)DepositorInsertnucA
UseTagsHis and NSP4 signal secretion peptideExpressionBacterialMutationcodon optimized ecoli residues - 22-266PromoterT7Available sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
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CROPseq-Guide-Puro
Plasmid#86708PurposeMakes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.DepositorInsertEF1a-Puro-WPRE-hU6-gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
Plasmid#60903PurposeExpressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activationDepositorInsertCas9 dead
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro eGFP
Plasmid#140027PurposeTunable and temporal expression control of nuclear eGFPDepositorInsertEnhanced Green Fluorescent Protein
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-Lysosomes-20
Plasmid#56487PurposeLocalization: Lysosome Membrane, Excitation: 485, Emission: 507DepositorInsertLAMP1
UseTagssfGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits