Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 7161 - 7180 of 11510 results

  2. AbVec2.0-mIghg1

    Plasmid
    #127158
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotype
    Depositor
    Hedda Wardemann
    Insert
    Ighg1 (Ighg1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV IE1 gene promoter
    Availability
    Academic Institutions and Nonprofits only
    127158-plasmid-map-sequence-id-256842

    Enlarge

  3. AbVec2.0-mIghg2c

    Plasmid
    #127159
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2c isotype
    Depositor
    Hedda Wardemann
    Insert
    Ighg2c (Ighg2c Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV IE1 gene promoter
    Availability
    Academic Institutions and Nonprofits only
    127159-plasmid-map-sequence-id-256903

    Enlarge

  4. AbVec2.0-mIghg3

    Plasmid
    #127160
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG3 isotype
    Depositor
    Hedda Wardemann
    Insert
    Ighg3 (Ighg3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV IE1 gene promoter
    Availability
    Academic Institutions and Nonprofits only
    127160-plasmid-map-sequence-id-256904

    Enlarge

  8. pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

    Plasmid
    #127177
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector
    Depositor
    Phil Sharp
    Article
    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:
    Insert
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    Use
    Entry vector with transgene for gateway cloning
    Tags
    Flag- HA- Tandem Epitopes
    Expression
    Mutation
    Promoter
    None
    Availability
    Academic Institutions and Nonprofits only
    127177-plasmid-map-sequence-id-246566

    Enlarge

  10. PB-Neo-TetO-Cdk12_Nterm_FlagHa

    Plasmid
    #127179
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector
    Depositor
    Phil Sharp
    Article
    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:
    Insert
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    Use
    Piggybac transposase vector
    Tags
    Flag- HA- Tandem Epitopes
    Expression
    Mammalian
    Mutation
    Promoter
    TetO Promoter
    Availability
    Academic Institutions and Nonprofits only
    127179-plasmid-map-sequence-id-247939

    Enlarge

  15. ELL1 Streptag

    Plasmid
    #127278
    Purpose
    Expresses mouse ELL1 with streptactin tag in mammalian cells
    Depositor
    Christine Milcarek
    Insert
    ELL1 (NM_007924) (ELL Mouse)
    Use
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    127278-plasmid-map-sequence-id-250019

    Enlarge

  16. ELL1 CtoA

    Plasmid
    #127279
    Purpose
    Testing if ELL1 C to A mutation knock out the action on Myc as it does in human
    Depositor
    Christine Milcarek
    Insert
    ELL1 (NM_007924 plus CEYLHNK to AEYLHNK) (ELL Mouse)
    Use
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Mutation
    CEYLHNK to AEYLHNK by Q5 mutagenesis
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    127279-plasmid-map-sequence-id-249989

    Enlarge

  17. ELL3 Streptag

    Plasmid
    #127282
    Purpose
    Expresses mouse ELL3 with streptactin tag; does it degrade Myc?
    Depositor
    Christine Milcarek
    Insert
    ELL3 (NM_145973) (ELL3 Mouse)
    Use
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    127282-plasmid-map-sequence-id-250028

    Enlarge

  18. ELL3 Streptag CtoA

    Plasmid
    #127283
    Purpose
    Expresses mouse ELL3 with streptactin tag; To interrogate if the mutation decreases putative Myc activity
    Depositor
    Christine Milcarek
    Insert
    ELL3 (NM_145973) (ELL3 Mouse)
    Use
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Mutation
    CEYLHNK to AEYLHQK
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    127283-plasmid-map-sequence-id-250049

    Enlarge

  19. Myc var1

    Plasmid
    #127284
    Purpose
    Expresses Myc to see if degraded by ELLs
    Depositor
    Christine Milcarek
    Insert
    Myc (NM_001177352) (Myc Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    transcript variant 1
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    127284-plasmid-map-sequence-id-249994

    Enlarge

  20. Myc var1 K397R

    Plasmid
    #127285
    Purpose
    Expresses Myc with putative Ub site mutated
    Depositor
    Christine Milcarek
    Insert
    Myc (NM_001177352) (Myc Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K397R by Q5 mutagenesis
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    127285-plasmid-map-sequence-id-249995

    Enlarge

Showing: 7161 - 7180 of 11510 results