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Plasmid#210346PurposeExpresses SARS-CoV-2 NSP14 fused with Flag in mammalian cellsDepositorInsertSARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
UseTagsFlagExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIR-CMV-SCN2A-Variant-4-IRES-mScarlet
Plasmid#209410PurposeExpression of wild-type neonatal isoform of human SCN2A.DepositorInsertSCN2A Variant 4, stabilized (SCN2A Human)
UseTagsExpressionMammalianMutationIVS intron inserted after bp 4551 of the SCN2A OR…PromoterCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL410-BRN2p
Plasmid#110733PurposeLuciferase reporter for the human BRN2 promoterDepositorInsertBRN2 promoter luciferase (POU3F2 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Myc-NLRP3(R260W)
Plasmid#73956PurposeExpress c-Myc-tagged human NLRP3(R260W) in mammalian cellsDepositorInsertNLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
UseTagsc-mycExpressionMammalianMutationR260WPromoterCMV iEAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5E-3x125bp-mnx1
Plasmid#169043Purpose5' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer.DepositorInsertMnx1 enhancer (3x) (mnx1 Zebrafish)
UseGateway entry cloning vectorTagsExpressionMutationPromoter3xMnx1Available sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6410
Plasmid#47049PurposeBglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPlacUV5Available sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hMeCP2-GFP
Plasmid#48078PurposeMammalian expression of GFP tagged MeCP2-WTDepositorInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBabe EGFR(L858R/T790M)
Plasmid#32073DepositorInsertEGFR (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationL858R/T790MPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-PQBP1
Plasmid#25804DepositorInsertPQBP1 (PQBP1 Human)
UseTags6xHAExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-25BoxB
Plasmid#60817PurposeCloning vector, 25 repeats of 19 bp BoxB sequence from bacteriophage-λ, for RNA labeling. BoxB-RNA is bound by 22 amino acid RNA binding domain of λ-anti-terminator protein N, e.g. tagged with GFPDepositorInsert25x BoxB
UseSynthetic BiologyTagsExpressionMammalianMutation25 repeats of BoxB sequencePromoterCMVAvailable sinceNov. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgNCKAP1-2
Plasmid#210134Purposeknock out NCKAP1 in mammalian cellsDepositorInsertNck-associated protein 1 (NCKAP1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgNCKAP1-1
Plasmid#210133Purposeknock out NCKAP1 in mammalian cellsDepositorInsertNck-associated protein 1 (NCKAP1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
DPYSL2-pLJC2
Plasmid#189866PurposeExpresses human DPYSL2 (CRMP2) in mammalian cellsDepositorInsertDPYSL2 (DPYSL2 Human)
UseLentiviralTags3X FLAGExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CaMKIV-dCT
Plasmid#126422PurposePlasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)DepositorInsertCalcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRNA7-mCherry
Plasmid#62632Purposemammalian expression of human Alpha7 (CHRNA7) with mCherry fused into M3-M4 loopDepositorInserthAlpha7 (CHRNA7 Human)
UseTagsExpressionMammalianMutationmCherry inserted into M3-M4 loopPromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA-32Asp8
Plasmid#102617PurposeExpression of human RPA with aspartic acid substituted phosphorylation domainDepositorUseTagsExpressionBacterialMutationwith aspartic acid substituted phosphorylation do…PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-HaloTag-Puromycin
Plasmid#167208PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe EGFR(L861Q)
Plasmid#32068DepositorInsertEGFR (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationL861QPromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-CFP
Plasmid#193357PurposeSelf-replicating RNA vector expressing CFP fluorescent protein + PuroRDepositorInsertCFP
UseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoterT7Available sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-FNIP2
Plasmid#72294PurposeoverexpressionDepositorInsertFNIP2 (FNIP2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only