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Plasmid#141236PurposeAAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)DepositorInsertNES-jRGECO1a
UseAAV and Cre/Lox; Cre-offTags6xHIS tag and nuclear export signalExpressionMutationPromoterhuman elongation factor-1 alpha (EF-1 alpha)Available sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pATZ-BFP-HA-GFP11
Plasmid#177721PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
UseTagsGFP11, HA, and TagBFPExpressionBacterial and MammalianMutationE342K (ATZ mutant)PromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-TAZ S89A
Plasmid#179911PurposeEntry vector of TAZ S89A for gateway coloning.DepositorInsertWW Domain Containing Transcription Regulator 1 (WWTR1 Human)
UseEntry vectorTags3X Flag-tagExpressionMutationSerine 89 mutated to AlaninePromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RVR
Plasmid#108861PurposeLbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutationsDepositorInsertLbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations)
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationG548R, K554V and Y558R, Cpf1 is rice codon optimi…PromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron Renilla STOP 12xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA
Plasmid#104094PurposeExpresses TREAT reporter in mammalian cellsDepositorInsertRenilla luciferase
UseTags12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…ExpressionMammalianMutationPromoterTetCMVAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
H6SUMO-IntC-P53(304-393)
Plasmid#200316Purposebacterial expression of P53 TET-CTD for segmental labelingDepositorInsertp53 (304-393) (TP53 Human)
UseTagsHis6-SUMO and designed intein CExpressionBacterialMutationPromoterT7Available sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53(1-303)IntN
Plasmid#200312PurposeBacterial expression of P53 NTAD-DBD for segmental labelingDepositorInsertp53 (TP53 Human)
UseTagsHis6GB1tev and designed intein NExpressionBacterialMutationM133L, V203A, N239Y, N268DPromoterT7Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA
Plasmid#104095PurposeExpresses TREAT-siRNA reporter in mammalian cellsDepositorInsertRenilla luciferase
UseTags24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…ExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mClover3
Plasmid#105795PurposeExpression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mClover3ExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGPN-ACE2
Plasmid#187483PurposeExpression vector - ACE2DepositorInsertACE2 (ACE2 Human)
UseLentiviralTagsExpressionMutationPromoterEFSAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only