pcDNA6-E2F4CA-Myc
(Plasmid
#80428)
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PurposeMammalian expression of constitutively active chick E2F4
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 80428 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA6/V5-His-A2
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameE2F transcription factor 4
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SpeciesG. gallus (chicken)
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MutationT261E, T263E
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Entrez GeneE2F4
- Promoter CMV
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Tag
/ Fusion Protein
- Myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA6-E2F4CA-Myc was a gift from José María Frade (Addgene plasmid # 80428 ; http://n2t.net/addgene:80428 ; RRID:Addgene_80428) -
For your References section:
Nerve growth factor-induced cell cycle reentry in newborn neurons is triggered by p38MAPK-dependent E2F4 phosphorylation. Morillo SM, Abanto EP, Roman MJ, Frade JM. Mol Cell Biol. 2012 Jul;32(14):2722-37. doi: 10.1128/MCB.00239-12. Epub 2012 May 14. 10.1128/MCB.00239-12 PubMed 22586272
