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Plasmid#71609Purposemammalian expression of human SEPT9 i1 with a GFP fusion.DepositorInsertSeptin 9 (SEPTIN9 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAWH-largeT
Plasmid#133895PurposePositive control when used in combination with pBWH-p53. Negative control when used in combination with pBWH-Lam. Expression of Gal4AD-largeT hybrid protein. Homology regions for recombination with pBDepositorInsertLargeT
UseTagsExpressionYeastMutationPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO eNpHR 3.0-EYFP
Plasmid#26966PurposeAAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibitionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InserteNpHR 3.0
UseAAVTagsEYFPExpressionMammalianMutationER export at 3' end of gene. Trafficking sig…PromoterEF1alphaAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNPK-NLS-Vika
Plasmid#79969Purposeexpresses human codon-optimized Vika-recombinase in mammalian cellsDepositorInserthuman codon-optimized Vika sequence, NLS tagged
UseTagsNLSExpressionMammalianMutationPromoterPGKAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Plxnb1(L)-Fc-His
Plasmid#72126PurposeExpresses the entire extracellular region of the PlexinB1 protein (ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertPlxnb1 (Plxnb1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-mDlx-NLS-mRuby2
Plasmid#99130PurposeAn AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancerDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV8, and AAV9InsertNLS-mRuby2
UseAAVTagsExpressionMammalianMutationPromotermDLX5/6Available sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F4
Plasmid#55787PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)DepositorInsert3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hTGM3-Flag
Plasmid#180408Purposelentiviral transduction of human TGM3 (with a C-terminal Flag tag) geneDepositorInsertTGM3 (TGM3 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GNPTAB
Plasmid#78108PurposeMammalian expression of human GNPTAB with C terminal myc tagDepositorInsertGNPTAB (GNPTAB Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV_tKiMBI-T2A-caMEK
Plasmid#199578PurposeExpress tKiMBI and caMEK in an AAV vectorDepositorInsertsERK tdTomato-Kinase-Modulated Bioluminescent Indicator
constitutively active MEK
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Luciferase-pcw107-V5
Plasmid#64649Purpose(control) when used to produce lentivirus, express physiological levels of insertDepositorInsertn/a
UseLentiviralTagsV5ExpressionMutationPromoterPGKAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ihSyn1-tTA
Plasmid#99120PurposeAn AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTADepositorHas ServiceAAV1InserttTA
UseAAVTagsExpressionMammalianMutation*(see below)PromoterihSyn1Available sinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro Myr HA PIK3CA
Plasmid#12523DepositorInsertPIK3CA Myr (PIK3CA Human)
UseRetroviralTagsHA and MyrExpressionMammalianMutationMyristoylation. Constitutively active allele; I14…PromoterAvailable sinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRK5 BARK1 minigene
Plasmid#14695DepositorInsertBARK1 minigene (GRK2 Bovine)
UseTags3'UTR of human b-globinExpressionMammalianMutationBARK1 amino acids 495-689PromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs
Plasmid#86828PurposeExpression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequenceDepositorInsertLINC00673 (LINC00673 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tol2-CAG::Palmbow
Plasmid#158993PurposeMulticolor membrane-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing palmitoylated mCherry, mEYFP or mCerulean upon Cre recombination)DepositorInsertPalmbow
UseTagsThe default expressed EBFP2 is fused to H2B; oth…ExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV500 A-CREB
Plasmid#33371DepositorInsertCREB (Creb1 Mouse)
UseTagsFLAGExpressionMammalianMutationDominant Negative (see notes)PromoterCMVAvailable sinceDec. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin
Plasmid#199585PurposeExpress tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vectorDepositorInsertsERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
AkaLuc
Blasticidin
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB502-omega
Plasmid#74845PurposeGateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter2xCaMV35S enhancer and omegaAvailable sinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only