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Plasmid#163960PurposepEZT-BM vector with alpha3 gene after CMV promoter, published EM constructDepositorInsertHuman alpha3 nicotinic acetylcholine receptor subunit EM construct
UseBaculovirus (bacmam) production and mammalian exp…Tagsapocytochrome b(562)RIL (BRIL)ExpressionMammalianMutationAsn348-Ser402 replaced with apocytochrome b(562)R…PromoterCMVAvailable sinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 FLCN (WT) FLAG GFP
Plasmid#164978PurposeLentiviral vector for FLCN expression in mammalian cellsDepositorInsertFLCN (FLCN Human)
UseLentiviralTags1xFLAG-GFPExpressionMammalianMutationPromoterUBCAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a_FOXA2_P2A_Hygro_Barcode
Plasmid#120439PurposeBarcoded lentiviral vector to express FOXA2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertFOXA2 (FOXA2 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_ERG_P2A_Hygro_Barcode
Plasmid#120434PurposeBarcoded lentiviral vector to express ERG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertERG (ERG Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EphB4-Fc
Plasmid#200986PurposeMammalian expression plasmid for soluble EphB4 fused to IgG1 FcDepositorInsertEphB4 (EPHB4 Human)
UseTagsFc region of human IgG1ExpressionMammalianMutationSoluble ectodomain of EphB4PromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-EFNB2 (WT)
Plasmid#200975PurposeMammalian expression plasmid for wild type, myc-tagged EFNB2DepositorInsertEFNB2 (EFNB2 Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK322 (TOP2A-mAC (Hygro))
Plasmid#140653PurposeTOP2A tagging with mAID-CloverDepositorInsertTOP2A (TOP2A Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmRFP-N1 human cofilin S3A
Plasmid#50857Purposeexpresses constituvely active cofilin fused to mRFP in mammalian cellsDepositorInsertcofilin 1 S3A (CFL1 Human)
UseTagsmRFPExpressionMammalianMutationS3A constitutively activePromoterCMVAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 WT 2014 EBOV + Mucin-Like Domain
Plasmid#85794PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
UseTagsExpressionMammalianMutationPromoterCMV IEAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Blast-sgNT
Plasmid#138678PurposeExpresses a Non-targeting sgRNA and Cas9DepositorInsertsgNT
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-BHLHE40
Plasmid#110154PurposeExpression of human BHLHE40 in mammalian cellsDepositorInsertBHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmRFP-N1 human cofilin S3E
Plasmid#50858Purposeexpresses psuedo-phosporylated, non-activatable cofilin fused to mRFP in mammalian cellsDepositorInsertcofilin 1 S3E (CFL1 Human)
UseTagsmRFPExpressionMammalianMutationS3E psuedo-phosporylated, non-activatablePromoterCMVAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMK321 (TOP2A-mAC (Neo))
Plasmid#140652PurposeTOP2A tagging with mAID-CloverDepositorInsertTOP2A (TOP2A Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDipA-TEV-His12
Plasmid#137042Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66790.1 (BB_0418 Borrelia burgdorferi B31)
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
CR1-isoformB-CD4d3+4-bio
Plasmid#73099PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding CR1-isoformB with rat CD4d3+4-bioDepositorInsertCR1 isoform B (CR1 Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-DMD-Donor
Plasmid#60605PurposeKnock-in donor vector to insert Exon 44 in front of Exon 45 of human DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette flanked by loxP sequences.DepositorInsertHygromycin resistant gene
UseKnock-in donor vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
phROSA26-Tet-HA-JSAP1_WT
Plasmid#208772PurposeTetracycline inducible expression vector for HA-JSAP1_WT, used as a donor plasmid for HA-JSAP1_WT knock-in at the human ROSA26 locusDepositorInsertJSAP1 (MAPK8IP3 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP773_pLenti-6xHRBKET-sgRNA
Plasmid#211766PurposeMultiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selectionDepositorInsertgRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)
UseLentiviralTagsExpressionMutationPromoterpCMV and U6Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-PIPK1 gamma 87
Plasmid#22300DepositorInserthuman phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET-EIN-AlkBH5(66-292)
Plasmid#125732PurposePlasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.DepositorInsertAlkBH5(66-292) (ALKBH5 Human)
UseTags6xHis and EINExpressionBacterialMutationPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only