These results may also be relevant.
-
MT3
Plasmid#223331PurposeMapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cellsDepositorInsert3HA-EGFP-miniTurboID-OMP25 (Synj2bp Rat)
UseTags3HA-EGFP-miniTurboIDExpressionPlantMutationPromoterCaMV35SAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
MT4
Plasmid#223332PurposeMapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cellsDepositorInsert3HA-miniTurboID-EGFP-OMP25 (Synj2bp Rat)
UseTags3HA-miniTurboID-EGFPExpressionPlantMutationPromoterCaMV35SAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-flex-Unc5cHA-WPRE-SV40pA
Plasmid#205442Purposeproduction of AAV for cre-dependent expression of Unc5C, tagged with 3xHA at C-terminusDepositorInsertUnc5c (Unc5c Mouse)
UseAAVTags3xHAExpressionMutationPromotermini-CMV (with MVM small intron)Available sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV(WT)-mouse-full-length-CHMP3-HA
Plasmid#154175Purposeexpresses mouse CHMP3 in mammalian cellsDepositorInsertCHMP3 (Chmp3 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-mouse-CHMP3(147)-HA
Plasmid#154177Purposeexpresses mouse CHMP3(147) in mammalian cellsDepositorInsertCHMP3 (Chmp3 Mouse)
UseTagsHAExpressionMammalianMutationC-terminal truncation of CHMP3 at amino acid 147PromoterEF1alphaAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
AdMKP-3 S159AS197A
Plasmid#131523PurposeExpresses MKP-3 with S159A mutation and S197A mutation in mammalian cellsDepositorInsertMitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsNoExpressionMutationchanged Serine 159 to Alanine and Serine 197 to A…PromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Slc25a51-g1)-PGKpuroBFP-W
Plasmid#105028PurposeLentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFPDepositorInsertSlc25a51 (Slc25a51 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Slc25a51-g2)-PGKpuroBFP-W
Plasmid#105029PurposeLentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFPDepositorInsertSlc25a51 (Slc25a51 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330 Pten
Plasmid#59909PurposepX330 backbone expressing sgRNA targeting Pten to edit mouse Pten. Expresses Cas9 from CBh promoterDepositorInsertsgRNA targeting Pten (Pten Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9Pubi-H
Plasmid#66187Purposeexpression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotermaize ubiquitin promoter (Pubi)Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB-3xFlag-dCas13-mTET2CD
Plasmid#228234PurposeFor targeted tethering of the catalytic domain of mouse TET2 using dCas13DepositorInsertsUseCRISPRTagsExpressionMammalianMutationCD (cysteine-rich, dioxygenase domain) truncation…PromoterCAGAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
FRB-GFP-Gab2(Y604F/Y634F)
Plasmid#188659PurposeFRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to PheDepositorInsertGRB associated binding protein 2 (Gab2 Mouse)
UseTagsFRB and GFPExpressionMammalianMutationY604F and Y634FPromoterCMVAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9Pubi-B
Plasmid#66188Purposeexpression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotermaize ubiquitin promoter (Pubi)Available sinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Bhlhe40-3xFLAG-IRES-BFP
Plasmid#117263PurposeRetroviral overexpression of Bhlhe40 with a 3xFLAGDepositorInsertBhlhe40 (Bhlhe40 Mouse)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterLTRAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW/TOPO-ELK4
Plasmid#98617PurposeGateway entry TOPO TA cloning vector for ELK4DepositorInsertELK4 (Elk4 Mouse)
UseTopo ta cloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9pUbi-N
Plasmid#106331PurposeExpression of Cas9 in plants, ZmUbi promoter, Kanamycin selectionDepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…PromoterZmUbiAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-shMcu-1
Plasmid#181868PurposeExpresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoterDepositorInsertmitochondrial calcium uniporter (Mcu Mouse)
UseAAV, Adenoviral, and Mouse TargetingTagshrGFPExpressionMammalianMutationPromoterU6Available sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MCU-EGFP
Plasmid#181866PurposeExpresses GFP-tagged mitochondrial calcium uniporter (MCU) under control of the CaMKIIa promoterDepositorInsertmitochondrial calcium uniporter (Mcu Mouse)
UseAAV and AdenoviralTagsEGFPExpressionMammalianMutationPromoterCaMKIIaAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pScalps_Puro_mTet2 catalytic domain
Plasmid#79554PurposeExpression of catalytic domain of mouse Tet2DepositorInsertTet2 (Tet2 Mouse)
UseLentiviralTagsMycExpressionMutationaa990-1912PromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
CRY2clust-mCherry-RANK-P2A-CIBNcaax
Plasmid#208613PurposeMammalian expression of CRY2clust-mCherry-RANK-P2A-CIBNcaax (Opto-RANKm)DepositorInsertCRY2clust-mCherry-RANK-P2A-CIBNcaax (Cry2 Mouse, Mustard Weed)
UseTagsCAAX and mCherryExpressionMammalianMutationPromoterCAGAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only
