K218Tc-3
(Plasmid
#1300)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1300 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepJC25S
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemutant HSP104
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Alt nameHSP104
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SpeciesS. cerevisiae (budding yeast)
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MutationK218T: A315T
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Entrez GeneHSP104 (a.k.a. YLL026W)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (destroyed during cloning)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
K218Tc-3 was a gift from Susan Lindquist (Addgene plasmid # 1300 ; http://n2t.net/addgene:1300 ; RRID:Addgene_1300) -
For your References section:
Subunit interactions influence the biochemical and biological properties of Hsp104. Schirmer EC, Ware DM, Queitsch C, Kowal AS, Lindquist SL. Proc Natl Acad Sci U S A 2001 Jan 30;98(3):914-9. Epub 2001 Jan 23. 10.1073/pnas.031568098 PubMed 11158570