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Showing: 1701 - 1750 of 147948 results

  1. mEmerald-FilaminA-N-9

    Plasmid
    #54098
    Purpose
    Localization: Actin/Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    FilaminA (FLNA Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    D2626H
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. TFORF2161

    Plasmid
    #141950
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GRHL1 (GRHL1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141950-plasmid-map-sequence-id-309802

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  10. pET-28a(+)::NL

    Plasmid
    #141289
    Purpose
    NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    NLUC
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    141289-plasmid-map-sequence-id-324904

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  13. MSGV Mouse Acceptor

    Plasmid
    #128176
    Purpose
    A TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR ref Biotechniques. 2015 Mar 1;58(3):135-9
    Depositor
    David Ott
    Article
    Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    MSCV
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128176-plasmid-map-sequence-id-252503

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  15. Lamp1-mScarlet-I

    Plasmid
    #98827
    Purpose
    In vivo visualization of lysosomes (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    Lamp1
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98827-plasmid-map-sequence-id-430619

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  16. pBECKP-km

    Plasmid
    #117235
    Purpose
    A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae
    Depositor
    Quanjiang Ji
    Article
    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii
    Insert
    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    117235-plasmid-map-sequence-id-227412

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  19. YFVdelNS1-Cre

    Plasmid
    #179952
    Purpose
    pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with Cre
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    YFV-17D lacking nonstructural protein 1 (NS1), but with Cre
    Use
    Transneuronal tracing
    Tags
    Expression
    Mutation
    Promoter
    SP6
    Available since
    Feb. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    179952-plasmid-map-sequence-id-354333

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  21. pcDNA3-Ii-OVA.II

    Plasmid
    #64597
    Purpose
    Expression of invariant chain fused to chicken ovalbumin 315-353 amino acids as model antigen for study of MHC class II presentation
    Depositor
    Sandra Diebold, Martin Zenke
    Article
    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.
    Insert
    Ovalbumin
    Use
    Tags
    Invariant chain
    Expression
    Mammalian
    Mutation
    Only amino acids 315-353 (class II epitopes)
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64597-plasmid-map-sequence-id-118016

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  25. 6BY9 (EHMT1)

    Plasmid
    #119956
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    EHMT1 (EHMT1 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    119956-plasmid-map-sequence-id-232626

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  31. dSaCas9-VPR

    Plasmid
    #205251
    Purpose
    In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495
    Depositor
    Rasmus Bak
    Article
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    dSaCas9-VPR
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    205251-plasmid-map-sequence-id-408439

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  37. CMV-SNAP-hCB1_IRES_EGFP

    Plasmid
    #223505
    Purpose
    Expression of CB1 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker
    Depositor
    James Frank
    Insert
    SNAP-tag-CNR1 and EGFP (CNR1 Synthetic, Human)
    Use
    Tags
    SNAP-tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    223505-plasmid-map-sequence-id-443299

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  48. pJR89

    Plasmid
    #140096
    Purpose
    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140096-plasmid-map-sequence-id-292016

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Showing: 1701 - 1750 of 147948 results