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Addgene

pAKgfplux1
(Plasmid #14083)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14083 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAKgfp1
  • Backbone size w/o insert (bp) 5681
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Bacterial luciferase
  • Alt name
    luxCDABE
  • Insert Size (bp)
    5866

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAKgfplux1 was a gift from Attila Karsi (Addgene plasmid # 14083 ; http://n2t.net/addgene:14083 ; RRID:Addgene_14083)
  • For your References section:

    Broad host range fluorescence and bioluminescence expression vectors for Gram-negative bacteria. Karsi A, Lawrence ML. Plasmid. 2007 May;57(3):286-95. doi: 10.1016/j.plasmid.2006.11.002 10.1016/j.plasmid.2006.11.002 PubMed 17207855