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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14081 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBBR1MCS4
- Backbone size w/o insert (bp) 4980
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameBacterial luciferase
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Alt nameluxCDABE
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Insert Size (bp)5818
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAKlux2.1 was a gift from Attila Karsi (Addgene plasmid # 14081 ; http://n2t.net/addgene:14081 ; RRID:Addgene_14081) -
For your References section:
Broad host range fluorescence and bioluminescence expression vectors for Gram-negative bacteria. Karsi A, Lawrence ML. Plasmid. 2007 May;57(3):286-95. doi: 10.1016/j.plasmid.2006.11.002 10.1016/j.plasmid.2006.11.002 PubMed 17207855