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Plasmid#114311PurposeTissue-specific expression of the tdTomato living color under the control of the Rat Insulin 2 promoter (RIP)DepositorInserttdTomato
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-DDX53
Plasmid#222240PurposeExpress DDX53 protein in bacteriaDepositorInsertDDX53 (DDX53 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-LV_3CL
Plasmid#203507PurposeExpresses LV 3CL protease from a GAL promoter with a URA3 markerDepositorInsertLV 3CL protease
UseTagsExpressionYeastMutationPromoterGAL1Available sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-SHV_3CL
Plasmid#203473PurposeExpresses SHV 3CL protease from a GAL10 promoter with a URA3 markerDepositorInsertSHV 3CL protease
UseTagsExpressionYeastMutationPromoterGAL10Available sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-HHV-3_PR
Plasmid#203505PurposeExpresses protease domain of HHV-3 (VZV) from a GAL promoter with a URA3 markerDepositorInsertHHV-3 protease domain
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-NWV_3CL
Plasmid#203470PurposeExpresses NWV 3CL protease from a GAL10 promoter with a URA3 markerDepositorInsertNWV 3CL protease
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-HTLV_PR
Plasmid#203467PurposeExpresses HTLV protease from a GAL10 promoter with a URA3 markerDepositorInsertHTLV protease
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-Halotag-RfA
Plasmid#186377PurposeDestination vector for the expression of a fluorescent fusion of N-ter Halo tag and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-Snaptag-stop
Plasmid#186395PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Snap tag into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-Halotag-stop
Plasmid#186387PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Halo tag into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR_Wheat_live_Cas9
Plasmid#126880PurposeGateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and BpiI sites for sgRNA modules.DepositorInsertWheat_live_Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
EFR Y877F (17)
Plasmid#102415Purposefluorescent tagging. Plant expression of EFR Y877F (17)DepositorInsertEFR (EFR Mustard Weed)
UseTags6xHis and mgfp5ExpressionPlantMutationY877FPromoter35SAvailable sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
EFR Y875F (15)
Plasmid#102414Purposefluorescent tagging. Plant expression of EFR Y875F (15)DepositorInsertEFR (EFR Mustard Weed)
UseTags6xHis and mgfp5ExpressionPlantMutationY875FPromoter35SAvailable sinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_50_CA_repeat
Plasmid#65869PurposeCarries CA repeats of 50 nucleotides in length, an RBPMS targetDepositorInsertCA repeats 50 nucleotides in lngth
UseTagsExpressionMutationPromoterT7Available sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pProkR2.Cre.T2A.Venus
Plasmid#169014PurposeExpresses Cre under Prok receptor 2 minimal promoter in mammalian cellsDepositorInsertCre Recombinase
UseAAV and Cre/LoxTagsT2A and VenusExpressionMammalianMutationPromoterProkineticin Receptor 2Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCLX-PGK-hCDK4
Plasmid#114314PurposeConstitutive expression of the CDK4 proteinDepositorInsertCDK4 (CDK4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1-Cre-PGK-Puro
Plasmid#108543PurposeLentivirus vector expessing Cre under EF1a promoter and puromycin under PGK promoterDepositorInsertscre recombinase
Puromycin
UseTagsExpressionMutationPromoterEF1apha and PGKAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
IDG_TMC3_OE_1
Plasmid#161629PurposeOverexpresses TMC3DepositorInsertTMC3 (TMC3 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-ccdB-Blast
Plasmid#158560PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUbi_dCas9-BFP-KRAB-gRNA
Plasmid#221503PurposeCRISPRi machinery fish codon optimized expressing plasmidDepositorInsertdCas9-tagBFP-KRAB
UseFish expressionTagsExpressionMutationD10A and H839A point mutationsPromoterZebrafish Ubiquitin promoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only