pCLX-PGK-hCDK4
(Plasmid
#114314)
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PurposeConstitutive expression of the CDK4 protein
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 114314 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCLX
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCDK4
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SpeciesH. sapiens (human)
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Entrez GeneCDK4 (a.k.a. CMM3, PSK-J3)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Due to the role of the transgene in cell proliferation, lentivectors produced with human-tropic envelopes must be produced in BSL2+/BSL3 conditions.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCLX-PGK-hCDK4 was a gift from Patrick Salmon (Addgene plasmid # 114314 ; http://n2t.net/addgene:114314 ; RRID:Addgene_114314)