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Showing: 3411 - 3420 of 148405 results

  1. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Article
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Promoter
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    60847-plasmid-map-sequence-id-197511

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  4. Perox-RA

    Plasmid
    #209869
    Purpose
    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddRFP A
    Use
    Tags
    Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209869-plasmid-map-sequence-id-415405

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  7. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Maja Jagodic
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190728-plasmid-map-sequence-id-381333

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  9. P4HB-bio-His

    Plasmid
    #52069
    Purpose
    Expresses full-length Protein disulfide-isomerase precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    P4HB (P4HB Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52069-plasmid-map-sequence-id-80294

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Showing: 3411 - 3420 of 148405 results