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pAAV.U6.shRNA-Sdc1-1376

Plasmid

#195574

Purpose

Expresses shRNA to knockdown mouse Syndecan-1

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

shRNA, eGFP (Sdc1 Synthetic)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2-CAG::Cytbow

Plasmid

#158991

Purpose

Multicolor cytoplasmic cell labeling with a genome-integrating Tol2 transposon vector (randomly expressing 3 tdTomato, mCerulean or mEYFP upon Cre recombination)

Depositor

Jean Livet

Article

Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

Insert

Cytbow

Use

Tags

The default expressed EBFP2 is fused to H2B

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CK(0.4)-CRY2PHR-P2A-CIBN-mCherry-VAMP2

Plasmid

#178576

Purpose

AAV vector for CK(0.4)::Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CRY2PHR-P2A-CIBN-mCherry-VAMP2

Use

AAV

Tags

Expression

Mutation

Promoter

CamKIIa

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3

Plasmid

#108912

Purpose

Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Has Service

AAV9

Insert

ChroME-ST

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pUC-fFuCas9-HTBNLS-hph

Plasmid

#121092

Purpose

Multigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 system

Depositor

Xiao-Jun Ji

Article

Shi et al ACS Synth Biol. 2019 Feb 15;8(2):445-454

Insert

Cas9 and hygromycin resist gene

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

gpdA;trpC

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-FingR-PSD95-mGL

Plasmid

#217533

Purpose

Genetically encoded fluorescent probe for labelling endogenous excitatory synapses on PV interneurons in vivo

Depositor

Peter Scheiffele

Article

Okur et al bioRxiv 2023

Insert

ZnF-FingR-PSD95-mGreenLantern (Dlg4 Mouse)

Use

AAV

Tags

mGreenLantern

Expression

Mammalian

Mutation

Promoter

S5E2 enhancer

Available since

April 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-mGrn-myc

Plasmid

#178944

Purpose

Construct for expressing murine progranulin (Grn) in AAV

Depositor

Erik Roberson

Article

Arrant et al Brain. 2017 May 1;140(5):1447-1465. doi:

Insert

Grn (Grn Mouse)

Use

AAV

Tags

Myc

Expression

Mutation

Promoter

CBA

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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tdp43-EGFP construct3

Plasmid

#28196

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa170-414 only

Promoter

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-Syn1-fDIO-mScarlet-KASH-2a-dCre

Plasmid

#203839

Purpose

Flp-dependent enzymatically inactive Cre and mScarlet-KASH expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dCre

Use

AAV

Tags

mScarlet-KASH-2A

Expression

Mutation

Enzymatically inactive Cre

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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PZac2.1 hSyn1-Lck-13xLinker-BioID2-BioID2-HA

Plasmid

#176854

Purpose

Expresses membrane bound biotin ligase, BioID2, in neurons

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Lck-13xLinker-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Mutation

Promoter

hSyn1

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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tdp43-EGFP construct2

Plasmid

#28195

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa86-414 only

Promoter

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
pLenti-CamKIIα-CRY2PHR-P2A-CIBN-mCherry-VAMP2

Plasmid

#178574

Purpose

Lentiviral vector for CamKIIα::Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CRY2PHR-P2A-CIBN-mCherry-VAMP2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CaMKIIa

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_SpikeyGi2_kv2.1

Plasmid

#195313

Purpose

Genetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, in AAV vector under the neuronal-specific promoter hSyn

Depositor

Vincent Pieribone

Article

Platisa et al Nat Methods. 2023 Mar 27. doi: 10.1038/s

Insert

SpikeyGi2

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pyc111f

Plasmid

#158780

Purpose

ribosome-tagged, Cre-dependent GCaMP for soma localization

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6f-RPL10a

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-synaptoPAC-minWPRE

Plasmid

#153085

Purpose

Expresses synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

Depositor

Dietmar Schmitz

Article

Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

Inserts

bPAC(WT)
Synaptophysin (Syp Rat)

Use

AAV

Tags

His tag, mScarlet, and myc tag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-PIG-N3FLAG-TAF10

Plasmid

#105990

Purpose

retrovirally express mouse TAF10 with 3*FLAG tag at N terminal, GFP marker and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

FLAG tagged TAF10 (Taf10 Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-GFAP-CRY2PHR-P2A-CIBN-mCherry-VAMP2

Plasmid

#178575

Purpose

Lentiviral vector for GFAP::Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CRY2PHR-P2A-CIBN-mCherry-VAMP2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

GFAP

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pyc111m

Plasmid

#158779

Purpose

ribosome-tagged, Cre-dependent GCaMP for soma localization

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6m-RPL10a

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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tdp43-EGFP construct5

Plasmid

#28198

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa257-414 only

Promoter

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
px459-TDP 43 sgRNA2

Plasmid

#133763

Purpose

Expresses Cas9 and human TDP-43 sgRNA

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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