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pAC8-HUWE1-HECTonly

Plasmid

#187119

Purpose

insect cell expression of HUWE1 HECT domain

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

Strep

Expression

Insect

Mutation

delta1-3992

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_UBA/UIM-mut

Plasmid

#187151

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

M1328A/F1330A/A1376G/S1382A

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_UBM-mut

Plasmid

#187149

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

L2976A/P2977A

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_UBA-mut

Plasmid

#187145

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

M1328A/F1330A

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_BH3-4E

Plasmid

#187143

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

V1976E/V1780E/L1983E/M1987E

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_delta169-189

Plasmid

#187134

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

delta169-189

Promoter

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_delta3896-3951

Plasmid

#187132

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

delta3896-3951

Promoter

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_I3969A/F3982A

Plasmid

#187128

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

I3969A/F3982A

Promoter

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_YH/GG_delta3291-3298

Plasmid

#187126

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

Y355G/H356G, delta3291-3298

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_delta3291-3298

Plasmid

#187124

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

delta3291-3298

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_YH/GG

Plasmid

#187122

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Tags

Expression

Mutation

Y355G/H356G

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GFP-IRES-mSNCA(1-95)

Plasmid

#185716

Purpose

AAV expression of GFP and C-terminal truncated (1-95 amino acid) mouse α-Synuclein from hSyn promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

synuclein, alpha (Snca Mouse)

Use

AAV

Tags

Expression

Mutation

deleted AA 95-144

Promoter

hSyn

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-Cyp26a1

Plasmid

#185563

Purpose

AAV construct to ubiquitously express mouse Cyp26a1

Depositor

Connie Cepko

Article

Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

Insert

Cyp26a1 (Cyp26a1 Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-dCys-T46C

Plasmid

#110543

Purpose

Bacterial expression of E.coli DHFR C85A/C152S/T46C mutant

Depositor

Stephen Benkovic

Article

Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

Insert

ecDHFR

Use

Tags

Expression

Bacterial

Mutation

T46 mutated to C46, C85 mutated to A85, C152 muta…

Promoter

T7

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-dCys-L54C

Plasmid

#110542

Purpose

Bacterial expression of E.coli DHFR C85A/C152S/L54C mutant

Depositor

Stephen Benkovic

Article

Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

Insert

ecDHFR

Use

Tags

Expression

Bacterial

Mutation

L54 mutated to C54, C85 mutated to A85, C152 muta…

Promoter

T7

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET22-HT/purC

Plasmid

#73623

Purpose

Produces Escherichia coli 5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase (PurC), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

phosphoribosylaminoimidazolesuccinocarboxamide synthase (purC Escherichia coli str. K-12 substr. MG1655)

Use

Tags

His6

Expression

Bacterial

Mutation

Contains multiple silent mutations (E179, G185, G…

Promoter

T7

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

Plasmid

#110854

Purpose

Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

sgRNA scaffold with spacer

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

U6

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pPalmitoyl-mTurquoise2

Plasmid

#36209

Purpose

In vivo visualization of the membrane (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

p63(1-29) (ARHGEF25 Human)

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-ABERA-P2A-Puro

Plasmid

#112675

Purpose

Lentivirus for constitutive expression of ABE7.10 RA (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

ABERA

Use

Lentiviral

Tags

FLAG

Expression

Mutation

D10A

Promoter

EF1s

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCRISPR-HOT-P2A-Clover-BlastR

Plasmid

#138568

Purpose

Universal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ

Depositor

Hans Clevers

Article

Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

Insert

P2A-clover-BlastR

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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