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PurposeIn vivo visualization of the membrane (can be used for colocalization studies)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 36209 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmTurquoise2-N1
- Total vector size (bp) 4765
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep63(1-29)
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Alt namepalmitoylation sequence
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SpeciesH. sapiens (human)
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Insert Size (bp)87
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GenBank IDNM_182947
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Entrez GeneARHGEF25 (a.k.a. GEFT, p63RhoGEF)
- Promoter CMV
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Tag
/ Fusion Protein
- mTurquoise2 (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer AGGTCTATATAAGCAGAGC (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPalmitoyl-mTurquoise2 was a gift from Dorus Gadella (Addgene plasmid # 36209 ; http://n2t.net/addgene:36209 ; RRID:Addgene_36209) -
For your References section:
Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen L, Hink MA, van Weeren L, Gadella TW Jr, Royant A. Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/ncomms1738. 10.1038/ncomms1738 PubMed 22434194