pDONR221-HUWE1_BH3-4E
(Plasmid
#187143)
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PurposeEntry clone
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 187143 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDONR221
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Vector typeEntry clone
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHUWE1
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SpeciesH. sapiens (human)
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Insert Size (bp)13122
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MutationV1976E/V1780E/L1983E/M1987E
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Entrez GeneHUWE1 (a.k.a. ARF-BP1, HECTH9, HSPC272, Ib772, LASU1, MRXST, MULE, URE-B1, UREB1)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221-HUWE1_BH3-4E was a gift from Eric Fischer (Addgene plasmid # 187143 ; http://n2t.net/addgene:187143 ; RRID:Addgene_187143) -
For your References section:
Solenoid architecture of HUWE1 contributes to ligase activity and substrate recognition. Hunkeler M, Jin CY, Ma MW, Monda JK, Overwijn D, Bennett EJ, Fischer ES. Mol Cell. 2021 Sep 2;81(17):3468-3480.e7. doi: 10.1016/j.molcel.2021.06.032. Epub 2021 Jul 26. 10.1016/j.molcel.2021.06.032 PubMed 34314700
