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Plasmid#112829Purposetransient/stable overexpressionDepositorInsertLL5BIP (2410002F23Rik Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pALPS puro miR30-STAT1
Plasmid#117160PurposeTarget site ATATCCAGTTCCTTTAGGGCCDepositorInsertshRNA targeting miR30
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-TTF-1-HDD
Plasmid#74991PurposeRetroviral vector holding human TTF-1 (NKX2-1) with the homeodomain deletedDepositorInsertTTF-1 HDD mutant (NKX2-1 Human)
UseRetroviralTagsNoneExpressionMammalianMutationHDD = homeodomain deletedPromoterAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
PITX3-2A-eGFP-PGK-Puro
Plasmid#31943DepositorInsertPITX3 (PITX3 Human)
UseTargeting donorTagseGFPExpressionMutationPromoter-Available sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
tet pLKO.1-shCSE1L v1 puro
Plasmid#192345PurposeLentiviral expression vector for an inducible shCSE1L v1DepositorInsertshCSE1L v1 (CSE1L Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
tet pLKO.1-shCSE1L v2 puro
Plasmid#192346PurposeLentiviral expression vector for an inducible shCSE1L v2)DepositorInsertshCSE1L v2 (CSE1L Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-5
Plasmid#112840Purposetransient/stable overexpressionDepositorInsertGrb2 (Grb2 Mouse)
UseTagsGFPExpressionMammalianMutationFull length Grb2PromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3RA-P2A-Puro
Plasmid#110839PurposeLentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)DepositorInsertBE3RA
UseLentiviralTagsExpressionMutationD10APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2_IRES_iRFP670-H2A-P2A-PuroR
Plasmid#190076Purposeexpress human ACE2 with selectable markers iRFP670 and puromycin resistance geneDepositorInsertACE2 (ACE2 Human)
UseTagsExpressionMammalianMutationPromoternoneAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Teton-puro-shFAK #1
Plasmid#198757Purposeconditional knockdown of FAKDepositorInsertshFAK #1 (PTK2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Teton-puro-shFAK #2
Plasmid#198758Purposeconditional knockdown of FAKDepositorInsertshFAK #2 (PTK2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe(puro) mouse Egr4
Plasmid#108105PurposeRetroviral vector for expression of mouse Egr4DepositorInsertEgr4 (Egr4 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro-luc (shRNA)
Plasmid#153962PurposeExpresses a shRNA against firefly luciferaseDepositorInsertshRNA against firefly luciferase (19-bp-long target)
UseLuciferase, RNAi, and RetroviralTagsN/AExpressionMammalianMutationPromoterU6 promoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-U6-sgRNA-CMV-puro
Plasmid#169450Purposebackbone for sgRNA expressionDepositorInsertsgRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV(shRNA)-Puro-U6-CCEPR
Plasmid#108277PurposeshRNA for CCEPR lncRNADepositorInsertCCEPR (CCEPR Human)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-Puro-shPP2A-Abeta
Plasmid#15250DepositorInsertPP2A regulatory subunit A, beta isoform (PPP2R1B Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3RA-PGK-Puro
Plasmid#110843PurposeLentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)DepositorInsertBE3RA
UseLentiviralTagsExpressionMutationD10APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro SCRIB P305L
Plasmid#88887PurposeSCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backboneDepositorInsertSCRIB (SCRIB Human)
UseRetroviralTagsExpressionMutationProline to Leucine mutation @ 305th amino acidPromoterMSCV promoterAvailable sinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shMKP1.v2 puro
Plasmid#87791PurposeExpresses an inducible short hairpin targeting human MKP1 sequenceDepositorInsertshMKP1 (DUSP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE tightAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-UBE3BΔIQ-HA-IRES-Puro
Plasmid#107350PurposeExpresses HA tagged UBE3B deletion of IQ domain in mammalian cells.DepositorInsertUBE3BdelIQ (UBE3B Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
MSCV Puro-SV40:GFP
Plasmid#68483PurposeRetrovirus expressing GFP driven by SV40 promoterDepositorInsertsPuromycin Resistance
GFP
UseRetroviralTagsExpressionMammalianMutationPromoterLTR and SV40Available sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-NLS-LSSmKate1
Plasmid#31873DepositorInsertNLS-LSS-mKate1
UseRetroviralTagsExpressionMammalianMutationK69Y/P131T/S148G/M167E/T183S/M196V compared to mK…PromoterAvailable sinceDec. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA Oct4A -12
Plasmid#50921PurposeU6 driven sgRNA targeting OCT4 isoform A -12 bp from TSSDepositorInsertOct4A -12 sgRNA (POU5F1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBlue-loxPuro-APEX-PML_LASA
Plasmid#140273Purposea targeting plasmid for APEX-PMLDepositorInsertAPEX2
UseMouse TargetingTagsExpressionMutationPromoterendogenousAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-5xE
Plasmid#91993PurposeExpresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824E, S828E, T830E, S831E, S834E (5xE)PromoterAvailable sinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro GFP-UVRAG
Plasmid#38267DepositorInsertUV radiation resistance-associated gene protein (UVRAG Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Nprl2-g1)-PGKpuroBFP-W
Plasmid#105037PurposeLentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFPDepositorInsertNprl2 (Nprl2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Nprl2-g2)-PGKpuroBFP-W
Plasmid#105038PurposeLentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFPDepositorInsertNprl2 (Nprl2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro RalA (long) wt
Plasmid#19717DepositorInsertV-ral simian leukemia viral oncogene homolog A (RALA Human)
UseRetroviralTagsExpressionMammalianMutationNOTE: long splice variant with 3bp deletion and 1…PromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro shPOLE1_1
Plasmid#160810PurposeExpress Dox repressible shPOLE1DepositorInsertshPOLE1_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO1-puro-U6-sgRNA-TGM2
Plasmid#69239PurposeU6 driven SpCas9 sgRNA expression for TGM2 siteDepositorInsertTGM2 sgRNA (TGM2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSicoR (Puro) p65 shRNA2
Plasmid#22508DepositorInsertp65 shRNA2 (Rela Mouse)
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuro-Flag-Pol b(K72A)
Plasmid#23258DepositorInsertDNA polymerase beta (K72A) (POLB Human)
UseTagsFlagExpressionMammalianMutationK72APromoterAvailable sinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Puro-FH-AGO2-5xA
Plasmid#91992PurposeExpresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]DepositorInsertAGO2 (AGO2 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationS824A, S828A, T830A, S831A, S834A (5xA)PromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro HA-Atg14*dCC
Plasmid#38266DepositorInsertautophagy related genes 14 (ATG14 Human)
UseRetroviralTags3xHAExpressionMammalianMutationchanged A618T, A621T, C624A and T627C for siRNA r…PromoterLTRAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HF1RA-PGK-Puro
Plasmid#110860PurposeLentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)DepositorInsertCas9-HF1RA
UseLentiviralTagsFLAGExpressionMutationR661A, Q695A, Q926A, and NLS sequence at the N-te…PromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL Y98N-pBabe-puro
Plasmid#19236DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Tyrosin 98 to AsparaginPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shIAPEz_2
Plasmid#185017PurposeshRNA mediated knockdownDepositorInsertERV_IAPEz
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shIAPEz_1
Plasmid#185016PurposeshRNA mediated knockdownDepositorInsertERV_IAPEz
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Anti-Neuroligin-3 [N110/29.4R]
Plasmid#114518PurposeMammalian Expression Plasmid of anti-Neuroligin-3 (Rat). Derived from hybridoma N110/29.4.DepositorInsertanti-Neuroligin-3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn3 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL Y112N-pBabe-puro
Plasmid#19238DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Tyrosin 112 to AsparaginPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-Puro HLAG-2B-sgRNA
Plasmid#182552PurposeCas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G geneDepositorInserthSpCas9-2A-Puro-HLAG-2B-sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterCbhAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL Y112H-pBabe-puro
Plasmid#19237DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationchanged Tyrosin 112 to HistidinPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO3_AH
Plasmid#148855PurposeMammalian Expression of HsAGO3-sgRNADepositorInsertHsAGO3-sgRNA (AGO3 S.pyogenes)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMP-puro-Flag-HA-NR6A1
Plasmid#46833DepositorInsertNR6A1 (Nr6a1 Mouse)
UseRetroviralTagsFlag-HA, plus 8-amino acid residue linkerExpressionMammalianMutationNonePromoterAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
Myog'-2A-mCherryNLS-PGK-Puro
Plasmid#69548PurposeDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.DepositorInsertsUseMouse TargetingTags2x nuclear localization signal and PVT1-2A "…ExpressionMutationSilent V205V mutation in myogenin gene to prevent…PromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMY-MCS-P2A-PuroR-HA
Plasmid#163352PurposeRetroviral backbone for in-frame cloning of a gene of interest and a P2A separated Puromycin selection markerDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
OCT4 GFP puro Donor 2
Plasmid#22210DepositorInsertSA-OCT-GFP-2APuro-PA (POU5F1 Human)
UseTagsExpressionMutationPromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti X2 Puro Empty (w609-1)
Plasmid#20957Purpose3rd gen lentiviral RNAi empty control vector, 3’LTR insertion, PuroDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-M2rtTA-T2A-Puro
Plasmid#169175PurposepiggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174DepositorInsertM2rtTA-T2A-Puro
UsePiggybac transposonTagsExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only